Thalassemia StripAssays®
4Pages

Thalassemia StripAssays® The easy way to optimize thalassemia screening using established innovations in diagnostics Thalassemia Assays Key to efficient screening Thalassemias are a major public health problem, particularly in Mediterranean countries, the Middle East, Asia, India and parts of Africa. For the large majority of affected individuals there is only supportive management but no ultimate cure. Health authorities therefore focus on prevention programs based on carrier and premarital screening. Since only a few α- and β-globin alleles are prevalent in each at-risk population, large-scale screening programs are feasible but require simple and automated test procedures. In healthy adults 97-98% of total hemoglobin (Hb) is HbA, consisting of two α-globin and two β-globin olypeptides (α 2β2). p Abnormalities in the structure and synthesis of both globin chains lead to an imbalance causing the two main types of halassemia: t α-thalassemia and β-thalassemia. The loss of one of the two α-globin alleles (-α) on chromosome 16 causes α+-thalassemia, whereas α 0-thalassemia is due to i nactivation of both α-globin alleles (--). Two groups of β-globin mutations are distinguished, depending on whether they lead to a reduction (β +) or an absence (β 0) of β-globin synthesis. In many regions, α- and β-thalassemia coexist with a variety of d ifferent structural Hb variants. These complex interactions give rise to an extremely wide spectrum of clinical phenotypes. urthermore, F an inherited increase of γ-globin expression in patients can artially p compensate for the lack of normal β-globin chain ynthesis in s β-thalassemia as well as sickle cell disease and thereby can a meliorate the clinical phenotype of both disorders. ViennaLab offers the globally applicable α-globin StripAssay®, three tailored β-globin StripAssays® for the Mediterranean area (MED), India & Middle East (IME) and Southeast Asia (SEA), as well as the β-Thal Modifier StripAssay® β-globin MED

Mutations covered by ViennaLab p-Globin StripAssays®

Mutations covered by the ViennaLab a-Globin StripAssay® using two separate teststrips (A/B) per sample

I=1X11X1/\ /\5 ESTABLISHED INNOVATIONS IN DIAGNOSTICS The ViennaLab Thalassemia StripAssays® meet customer requirementsRequirement ViennaLab’s offer Easy Three simple steps. 6 h. Done. Reliable Probes for variants and controls combined on one teststrip. Versatile Automated or manual processing. Incubator.Thermocycler. Shaker. That is all you need. Software for interpretation of results is optional. The ViennaLab a- / (3-Globin and (3-Thal Modifier StripAssays® combine all these requirements. Thalassemia StripAssays®: • are based on reverse-hybridization of biotinylated PCR products •...