LAMP Designer Software Design Primers for Loop Mediated Isothermal Amplification LAMP Designer retrieves batches of sequences directly into the program from GenBank. Performs BLAST search and avoids cross homologies while designing primers. Supports Local and Desktop BLAST options for using local custom/unpublished databases. Includes a sequence view with primers marked on it. Calculates Tm using nearest neighbour thermodynamic theory and highly accurate SantaLucia values. LAMP Designer designs efficient primers for Loop-Mediated Isothermal Amplification assays, that amplify DNA and RNA sequences at isothermal conditions, eliminating the necessity of a PCR setup. The technology relies on autocycling and DNA polymerase mediated strand displacement DNA synthesis, amplifying a few copies of DNA to 109 in as little as 30 minutes. Reverse transcription coupled LAMP can be applied for amplification of RNA sequences. LAMP employs six specially designed primers that recognize eight distinct regions in the target DNA. Hybridization of the six primers to the target DNA is a very crucial step for the efficiency of LAMP. The design of these six primers is therefore critical for a successful assay. Exports a publication quality report for the assays designed for sharing with colleagues. Organizes data in project format and maintains a local database of sequence information and search results. Supported on latest operating system, Windows 7. Avoid Cross Homologies LAMP Designer automatically interprets BLAST search results and avoids those regions to design primers that have significant cross homologies with the database. Verification BLAST The primers can be BLAST searched against a database to verify their specificity. Multiplex LAMP Primer Set The primers designed for a sequence can be checked for multiplexing. The free energies of the most stable cross homologies between the primers designed, is displayed by the program. Export Result The designed primer sets, along with their properties, can be exported in standard csv or excel formats. Data & Database Management Multiple projects can be created. Data of multiple experiments can be easily managed by creating a separate project for each experiment. It maintains a local database for sequence information and search results. Free 7 day Trial Activate the program following these easy steps: Install and launch LAMP Designer - Click Evaluate on the first window that opens - Enter the evaluation key requested from us. OptiGene Limited Unit 5 Blatchford Road Horsham West Sussex RH13 5QR Tel: +44 (0) 1403 274980 Fax: +44 (0) 1403 271017 www.optigene.co.uk [email protected]