Vivaspin® 4 and Vivaspin® 6Your Source for Clinical & Biological Concentrators Call us at 800-456-4633Concentration & Purification of Clinical Biological Samples Vivaspin® ultrafiltration concentrators are disposable devices for biological samples. Vivaspin® 4 is suitable for sample volumes up to 4 ml while Vivaspin® 6 can handle samples as much as 6 ml. The patented vertical membrane design and thin channel filtration chamber (US 5,647,990) minimizes membrane fouling and provides high speed concentrations. Vivaspin® devices are for single use and Vivaspin® 4 devices are intended for in vitro diagnostic (IVD) applications. Some Vivaspin® 6 products are also intended for IVD applications. Vivaspin® Applications — Concentration of urine prior to electrophoresis for diagnosis of multiple myeloma and amyloidosis. — Concentration of spinal fluid prior to electrophoresis for diagnosis of meningitis and multiple sclerosis. — Concentration of bacterial antigens in urine (Legionella, Pneumonia, Streptococcus B) prior to immunoassay Technical Specifications Concentrator Capacity Vivaspin® 4 Vivaspin® 6 Fixed angle rotor 4 ml 6 ml Swinging bucket rotor 4 ml 6 ml (membrane & support) Dead stop volume** 20 pl 30 pl **Dead stop volume may vary depending on sample, temperature and rotor Polycarb. Polycarb. Polyprop. Polycarb. Polycarb. Polyprop. Materials of Construction Body Filtrate vessel Concentrator cap Membrane * Polycarb. = Polycarbonate, Polyprop. = Polypropylene * See other side for membrane descriptions Centrifuge Rotor cavity To fit 15 ml To fit 15 ml conical conical bottom bottom tubes tubes Fixed Rotor - Minimum Angle 25o 25o Fixed Rotor - Max. Speed 8,000 g 8,000 g ** **lower g force for these membranes: Features Benefits Vertical membrane design Fast filtration rates. Integrated dead stop Reduces risk of concentration to dryness. Low binding materials High sample recovery. Wide range of MW cutoffs Choose MW cutoff for your specific application. Large Vivaspin® family Can process samples from 100 pl to of devices 20 ml with similar methodology. Swinging Bucket -Max. Speed 4,000 g 4,000 g Pipettes for Sample Delivery & Recovery Fixed or variable volume may be used. For maximum recovery, a thin gel loader type is recommended.

Membrane Selection Guide Polyethersulfone (PES) A good general purpose membrane for most solutions. Has low fouling characteristics with very good flow rates. Tolerates a broad pH range (from 1 to 9). Good hydrophilic properties with very low non-specific binding. Cast without a membrane support that could bind filtered solutes. Use when recovery of filtrate is most important (free drug or hormone testing). Similar to regenerated cellulose but with better performance characteristics and low protein binding. Good choice for concentration and desalting of proteins that may bind to other membranes...