Vivaspin®500 and Vivaspin®2

Product catalog summary

Overview
Vivaspin® ultrafiltration concentrators are disposable devices designed for concentrating biological samples. They are available in different models, such as Vivaspin® 500 for sample volumes of 100-500 μl and Vivaspin® 2 for up to 2 ml. The devices feature a patented vertical membrane design that minimizes fouling and enhances concentration speed.

Applications

Concentration of spinal fluid for diagnosing meningitis and multiple sclerosis.
Concentration of antigens in urine and serum for immunoassays.
Protein removal from serum and cell/tissue lysates.
Buffer exchange for proteomic peptide samples.
Sample preparation for mass spectrometry.

Features and Benefits

Vertical membrane design ensures fast filtration rates.
Integrated dead stop prevents over-concentration.
Low binding materials ensure high sample recovery.
Available in a wide range of molecular weight cutoffs for specific applications.
Large family of devices can process samples from 100 μl to 20 ml.

Technical Specifications

Vivaspin® 500: Capacity of 0.5 ml with fixed angle rotor.
Vivaspin® 2: Capacity of 2.0 ml with fixed angle rotor and 3.0 ml with swinging bucket rotor.
Materials: Polycarbonate body and cap, polypropylene filtrate vessel, and various membrane types (PES, CTA, HY).

Performance Characteristics

Concentration times and sample recovery rates vary based on molecular weight cutoff and membrane type.
Example: Aprotinin (6,500 MW) with 3,000 MWCO PES membrane achieves 96% recovery in 30 minutes at 12,000 g.

Ordering Information

Available in various molecular weight cutoffs ranging from 2,000 to 1,000,000 MWCO.
Pack sizes of 25 or 100 units are available for different membrane types.

Contact Information
Manufactured by Sartorius Stedim Lab Ltd., with North American distribution by Vivaproducts. For pricing and online ordering, visit www.vivaproducts.com.

Catalog excerpts

Vivaspin® 500 and Vivaspin® 2Your Source for Clinical & Biological Concentrators Call us at 800-456-4633Concentration & Purification of Clinical Biological Samples **Dead stop volume may vary depending on sample, temperature and rotor Polycarb. Polycarb. Polyprop. Polycarb. Polycarb. Polycarb. Equipment Required Centrifuge Rotor cavity Vivaspin® ultrafiltration concentrators are disposable devices for biological samples. Vivaspin® 500 is suitable for sample volumes from 100-500 pl while Vivaspin® 2 can handle samples up to 2 ml. The patented vertical membrane design and thin channel filtration chamber (US 5,647,990) minimizes membrane fouling and provides high speed concentrations. Vivaspin® Applications — Concentrate spinal fluid prior to electrophoresis for diagnosis of meningitis and multiple sclerosis. — Concentrate antigens in urine & serum (Legionella, Pneumonia, Streptococcus B, Coccidioides) prior to immunoassays. — Protein removal from serum & cell / tissue lysates. — Exchange buffers for proteomic peptide samples. — Prepare samples for mass spectrometry. Features Benefits Vertical membrane design Fast filtration rates. Integrated dead stop Reduces risk of concentration to dryness. Concentrator Capacity Vivaspin® 500 Vivaspin® 2 Fixed angle rotor 0.5 ml 2.0 ml Swinging bucket rotor N/A 3.0 ml (membrane & support) Dead stop volume** 5 pl 8 pl Materials of Construction Body Filtrate vessel Concentrator cap Membrane * Polycarb. = Polycarbonate, Polyprop. = Polypropylene * See other side for membrane descriptions conical conical bottom bottom tubes tubes Fixed Rotor - Minimum Angle Fixed Rotor - Max. Speed Swinging Bucket -Max. Speed Low binding materials High sample recovery. Wide range of MW cutoffs Choose MW cutoff for your specific application. Large Vivaspin® family Can process samples from 100 pl to of devices 20 ml with similar methodology. Pipettes for Sample Delivery & Recovery Fixed or variable volume may be used. For maximum recovery, a thin gel loader type is recommended.

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Membrane Selection Guide Polyethersulfone (PES) A good general purpose membrane for most solutions. Has low fouling characteristics with very good flow rates. Tolerates a broad pH range (from 1 to 9). Good hydrophilic properties with very low non-specific binding. Cast without a membrane support that could bind filtered solutes. Use when recovery of filtrate is most important (free drug or hormone testing). Similar to regenerated cellulose but with better performance characteristics and low protein binding. Good choice for concentration and desalting of proteins that may bind to other membranes...

Open the catalog to page 2

Vivaspin®500 and Vivaspin®2

Vivaspin®500 and Vivaspin®2

Catalog excerpts

All Vivaproducts, Inc. catalogs and technical brochures