Vivaspin® 500 and Vivaspin® 2Your Source for Clinical & Biological Concentrators Call us at 800-456-4633Concentration & Purification of Clinical Biological Samples **Dead stop volume may vary depending on sample, temperature and rotor Polycarb. Polycarb. Polyprop. Polycarb. Polycarb. Polycarb. Equipment Required Centrifuge Rotor cavity Vivaspin® ultrafiltration concentrators are disposable devices for biological samples. Vivaspin® 500 is suitable for sample volumes from 100-500 pl while Vivaspin® 2 can handle samples up to 2 ml. The patented vertical membrane design and thin channel filtration chamber (US 5,647,990) minimizes membrane fouling and provides high speed concentrations. Vivaspin® Applications — Concentrate spinal fluid prior to electrophoresis for diagnosis of meningitis and multiple sclerosis. — Concentrate antigens in urine & serum (Legionella, Pneumonia, Streptococcus B, Coccidioides) prior to immunoassays. — Protein removal from serum & cell / tissue lysates. — Exchange buffers for proteomic peptide samples. — Prepare samples for mass spectrometry. Features Benefits Vertical membrane design Fast filtration rates. Integrated dead stop Reduces risk of concentration to dryness. Concentrator Capacity Vivaspin® 500 Vivaspin® 2 Fixed angle rotor 0.5 ml 2.0 ml Swinging bucket rotor N/A 3.0 ml (membrane & support) Dead stop volume** 5 pl 8 pl Materials of Construction Body Filtrate vessel Concentrator cap Membrane * Polycarb. = Polycarbonate, Polyprop. = Polypropylene * See other side for membrane descriptions conical conical bottom bottom tubes tubes Fixed Rotor - Minimum Angle Fixed Rotor - Max. Speed Swinging Bucket -Max. Speed Low binding materials High sample recovery. Wide range of MW cutoffs Choose MW cutoff for your specific application. Large Vivaspin® family Can process samples from 100 pl to of devices 20 ml with similar methodology. Pipettes for Sample Delivery & Recovery Fixed or variable volume may be used. For maximum recovery, a thin gel loader type is recommended.

Membrane Selection Guide Polyethersulfone (PES) A good general purpose membrane for most solutions. Has low fouling characteristics with very good flow rates. Tolerates a broad pH range (from 1 to 9). Good hydrophilic properties with very low non-specific binding. Cast without a membrane support that could bind filtered solutes. Use when recovery of filtrate is most important (free drug or hormone testing). Similar to regenerated cellulose but with better performance characteristics and low protein binding. Good choice for concentration and desalting of proteins that may bind to other membranes...