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PCR solutions from Avantor

PCR solutions from Avantor
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PCR solutions from Avantor

Product catalog summary
Introduction
The document introduces PCR solutions from Avantor, emphasizing the importance of Taq polymerase from Thermus aquaticus and the variety of PCR products available, including high fidelity enzymes and consumables.
Specifications
The VWR family of PCR thermal cyclers, engineered in Germany, adheres to ISO 9001 standards. They feature advanced capabilities such as gradient functions, precise temperature control, and remote operation. The XT96 and XTender96 models are noted for their flexibility and high throughput.
Procedures
The document details the use of VWR thermal cyclers for DNA amplification via PCR, highlighting the significance of precise temperature control and the use of Peltier elements for uniformity.
Norms and Standards
VWR thermal cyclers comply with national and international regulations, ensuring reliable and reproducible PCR results, with excellent temperature uniformity and control.
Recommendations
For optimal PCR results, the document recommends using VWR PCR Workstations to prevent contamination and VWR filter tips, along with VWR Collection dNTPs for purity and functionality.
Key Features of VWR Thermal Cyclers
  • Gradient PCR technology with up to 16 Peltier element control circuits.
  • Remote control via smartphone, tablet, or PC.
  • High-pressure lid options to prevent sample evaporation.
  • Compatibility with various PCR consumables and reagents.
Conclusion
Avantor is positioned as a partner for PCR solutions, offering a comprehensive range of products to meet diverse laboratory needs while ensuring cost-effectiveness and high-quality results.
XT96 Cycler and XTender96 Overview
The XT96 cycler is versatile, controllable via a primary XT96 cycler, PC, or mobile device, featuring a 7-inch TFT display and intuitive software. The XTender96 is a cost-effective replica unit without a built-in display.
Key Features
  • Flexible heated lid to prevent sample evaporation.
  • Innovative block design for high temperature uniformity and rapid heating/cooling rates.
  • Precise temperature control for optimal PCR results.
  • Optional gradient function for PCR optimization.
  • Intuitive programming with PCR-Wizard and quick setup options.
  • Data control via USB and Ethernet, with remote monitoring capabilities.
  • System tools for easy programming and auto-restart after power failure.
Specifications and Models
Various models of thermal cyclers, including UNO96, UNO96 HPL, and Doppio, are listed with specific features like block accuracy, homogeneity, and gradient temperature range.
PCR Workstation
The VWR PCR Workstation provides a UV decontamination environment for PCR sample preparation, featuring antimicrobial surfaces and UV air recirculation.
Reagents and Polymerases
The document details various DNA polymerases and master mixes optimized for specific PCR applications, including standard, hot start, and high-fidelity PCR.
Conclusion
The document highlights the advanced features and specifications of the XT96 cycler and related products, emphasizing their efficiency, flexibility, and ease of use in PCR applications.
DNA Polymerase Products Overview
The document provides detailed information on various DNA polymerase products offered by VWR, focusing on their applications, specifications, and formulations.
Specifications
  • Taq DNA Polymerase: Features a 5’ to 3’ exonuclease activity, ideal for TA cloning, thermostable, and suitable for routine applications.
  • Red Taq DNA Polymerase: Contains an inert red dye for visual recognition, optimal for TA cloning and standard amplification.
  • Taq Plus 2X Master Mix: Optimized for increased specificity and reduced contamination risk, ready to use for standard PCR applications.
  • TEMPase Hot Start DNA Polymerase: Offers higher specificity and sensitivity, suitable for low abundance targets and difficult templates.
Procedures and Applications
  • All polymerases are designed for standard amplification with an amplicon size of ≤ 5kb.
  • Master mixes are provided for ease of use, requiring only the addition of primers, template, and water.
  • Buffers are optimized to minimize the need for Mg²⁺ concentration and annealing temperature adjustments.
Reagents and Formats
  • Products are available in various formats, including glycerol-free options and master mixes with included dNTPs.
  • Storage conditions are specified as 20 °C for long-term and +4 °C for up to 6 months.
Recommendations
  • Choose the appropriate polymerase based on the specific application needs, such as TA cloning or high specificity requirements.
  • Utilize the provided buffers to enhance amplification signals and reduce optimization efforts.
PCR Solutions Overview
The document provides detailed information on various PCR solutions and reagents offered by VWR, including DNA polymerases, master mixes, clean-up reagents, extraction solutions, and DNA ladders.
1. DNA Polymerases and Master Mixes
  • Fast HiFi DNA Polymerase: A high-fidelity proofreading enzyme with >60X Taq fidelity, suitable for amplifying problematic DNA targets with low to high GC content.
  • Master Mix Variants: Available in 2X master mix format, including dNTPs and MgCl₂, for convenient handling and robust amplification.
2. PCR Clean-Up Reagents
  • ExoCleanUp FAST: A one-step enzymatic clean-up reagent for PCR products, improving downstream applications like DNA sequencing.
3. DNA Extraction Solutions
  • Fast Extract DNA Solution: Provides rapid extraction of PCR-ready DNA from mammalian tissues in eight minutes.
4. Genotyping Kits
  • Fast Extract Genotyping PCR Kit: Combines fast extraction DNA solution with Red Taq DNA polymerase 2X Master mix for efficient genotyping.
5. Buffers and Enhancers
  • PCR Buffers: Includes Key Buffer, Extra Buffer, and Combination Buffer, each tailored for specific PCR needs.
  • Betaine Enhancer: Effective for high GC-rich regions, reducing primer melting temperature.
6. DNA Loading Buffers and Ladders
  • Loading Dyes: Available in red, blue, and orange, suitable for various electrophoresis buffers.
  • DNA Ladders: Ready-to-use ladders for agarose and polyacrylamide gels, with mass-calibrated bands for DNA quantification.
Conclusion
VWR offers a comprehensive range of PCR solutions designed to enhance efficiency and accuracy in molecular biology applications.
PCR Consumables Overview
Avantor offers a comprehensive range of PCR consumables, including tubes, strips, and plates, designed to fit various PCR instruments.
Product Specifications
  • PCR Plates: Available in 96-well and 384-well formats, compatible with most thermal cyclers, featuring high-contrast alphanumeric indexing and ultra-thin walls for optimal heat transfer.
  • PCR Tubes and Strips: Available in various configurations, designed to fit most thermal cyclers, and certified free from DNase, RNase, and human DNA.
Compatibility and Customization
The consumables are designed to meet or exceed the specifications of original equipment manufacturers, ensuring broad compatibility with different thermal cyclers.
Environmental Considerations
Some products are noted for their low manufacturing impact and sustainable packaging.
Conclusion
Avantor's PCR consumables provide reliable and versatile solutions for PCR and qPCR applications.
Consumables and Sealing Films Overview
The document provides detailed information on various consumables and sealing films used in PCR and qPCR applications.
Specifications
  • Products include heat-resistant films, aluminum foils, and adhesive sealing films, each with specific temperature ranges and material compositions.
  • Films are certified free from DNase, RNase, and human genomic DNA.
Product Features
  • Heat-resistant films are available in polyester, polyolefin, and polypropylene materials.
  • Aluminum foils provide excellent vapor barriers and are easily pierceable for sample recovery.
  • Adhesive films are designed for easy application and removal.
Applications
  • Products are suitable for PCR, qPCR, storage, protein crystallization, and high-throughput screening.
  • Optically-clear films are recommended for real-time PCR applications.
Recommendations
  • Use optically-clear polyester films for real-time PCR.
  • For applications requiring piercing, aluminum foil films are recommended.
Key Data from Tables
  • Various films and foils are listed with their catalog numbers, temperature ranges, and material specifications.
PCR Racks
The reversible PCR racks are designed to hold various tube sizes, featuring 168 wells for 8-well or 12-well tube strips on one side and 40 wells for 0.5 ml tubes on the opposite side.
Microcentrifuge Tubes - High Performance
These tubes are made from USP Class VI PP, ensuring clarity and high strength, suitable for high g-force centrifugation, vortexing, and shaking.
Disposable Microcentrifuge Tubes
Made from USP Class VI PP, these tubes are suitable for light-sensitive substances and are RNase-, DNase-, and endotoxin-free.
Microcentrifuge Tubes with Socket Screw Caps
These tubes are ideal for secure sample handling, featuring silicone O-rings for a leakproof seal.
Microcentrifuge Tubes with Flat Screw Caps
Made from ultra-clear PP, these tubes allow easy content viewing and feature universal screw threads.
J.T.Baker® Tips
Suitable for robotic instruments, these tips are available in conductive and non-conductive versions, with or without filters.
Technical Specifications
1. Temperature Control: The device offers a heating speed of 5°C/min and a cooling speed ranging from 0.5 to 1.0°C/min below ambient.
2. Speed and Orbit: The speed range is 300 to 3000 min-1 with a speed accuracy of ±2%.
3. Physical Dimensions: The unit weighs 4.4 kg and measures 248×260×132 mm.
Product Variants
1. Thermo Shakers: Available with EU, UK, and CH plugs, with or without NIST traceable certificates.
2. Interchangeable Blocks: Various blocks are available for different tube sizes and applications.
Features and Applications
1. Safety and Control: The device includes a hot top warning and enhanced electronics for reliable temperature settings.
2. Certification: Models with NIST traceable certificates include a 3-point calibration.
Complementary Equipment
1. Thermal Shake Lite: Features a heating speed of 6.5°C/min, suitable for molecular biology and biochemistry applications.
Additional Services
Avantor Services offers equipment lifecycle management including installation, maintenance, calibration, and training.
Contact Information
VWR provides contact details for various regions for customer support and inquiries.
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Catalog excerpts

PCR solutions from Avantor-1

01. XT FAMILY OF PCR CYLERS: NEW APP CONTROL 02. PCR WORKSTATION NO ROOM FOR CONTAMINATION POLYMERASE: ULTRA-LOW ERROR RATE & HIGH PROCESSIVITY 04. VWR® FAST EXTRACT GENOTYPING PCR KIT FAST EXTRACT DNA SOLUTION & PCR KIT FOR GENOTYPING

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PCR solutions from Avantor-2

PCR SOLUTIONS FROM AVANTOR Welcome to the VWR range for PCR Who would have thought that a bacterium, discovered in 1966 in a hot spring in Yellowstone National Park, would become such a pivotal ingredient in the molecular biology revolution? From that gram-negative bacterium, Thermus aquaticus, came Taq, a thermostable DNA polymerase that survives the repeated melting and extension steps to enable the modern PCR reaction we know today. Taq, with its great processivity, is still the most widely used polymerase, and you will find a variety of formulations optimised to specific applications within...

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PCR solutions from Avantor-3

PCR SOLUTIONS FROM AVANTOR PCR machines for today’s most reliable, comfortable and sustainable way of running a PCR. Compartments that offer maximum protection during PCR sample preparation. High quality DNA polymerases, master mixes, dNTPs and buffers. Value for money PCR consumables in different formats and shapes to fit any need. Taq enzymes and master mixes Page 15-20 96-well XT96 cycler Pages 9-10 96- or 384-well Uno cyclers Page 12 PCR/qPCR tubes and strips Page 34 PCR/qPCR plates Page 32-33 and 35-37 Hot start enzymes and master mixes Page 21-22 High fidelity enzymes and master mixes Page...

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PCR solutions from Avantor-4

VWR® GLASSWARE VWR Thermal 96 Cycler XT Inside the VWR PCR Thermal Cycler XT96, a lot of new innovative features are in pl ace. PCR Solutions from Avantor | vwr.com

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PCR solutions from Avantor-5

VWR Family of Thermal Cyclers Thermal cyclers are used to amplify segments of DNA via the polymerase chain reaction (PCR). Also used to facilitate other temperaturesensitive reactions, these PCR machines or DNA amplifier devices can complete restriction enzyme digestion or rapid diagnostics. Each thermal cycle is equipped with high-performance components that ensure the formation of a homogenous tempered air cushion between the samples being combined. These instruments provide excellent temperature uniformity over the entire block and reproducible results regardless of the position of the inserted...

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PCR solutions from Avantor-6

Welcome to the VWR family of PCR thermal cyclers. Quality made in Germany Engineered and manufactured in Germany according to ISO 9001, calibrated and maintained according to NIST standards and backed with 30 years of cycler expertise and experience: VWR thermal cyclers will be your reliable workhorse ready to take on the daily challenges of PCR! More than this, being equipped with most sophisticated, state-of-the-art technology they will offer you an unmatched level of functionality and convenience. Explore their unique features and how you can benefit. VWR THERMAL CYCLERS FAMILY : More than...

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PCR solutions from Avantor-7

Temperature measurements executed using calibrated, external thermal probes. Blue: VWR Doppio. Grey: other leading system. NO DOUBT WHAT HAPPENS TO THE SAMPLE KEEP YOUR SAMPLE FREE OF CONTAMINATIONS ULTIMATE TEMPERATURE CONTROL While a VWR® PCR thermal cycler will ensure precise temperature control for highest yields of specific PCR products it cannot discriminate relevant templates from unwanted molecules introduced by contaminated aerosols or surfaces. Using VWR® filter tips and a VWR® PCR Workstation will help to avoid contaminations substantially. What makes a good thermal cycler? ‘Fastest...

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PCR solutions from Avantor-8

1. SIMPLE TO USE POWERFUL TO COMMAND EMULATION MODE & CO Combining advanced technology with an intuitive, easy to operate user interface VWR® thermal cyclers offer unmatched convenience and open-up for new possibilities. Depending on the cycler model features include: Up to date data and user management incl. GLP reporting, 500000+ program memory, USB/ Ethernet connectivity for quick data transfer/central cycler control, MP3 capability, Tm calculator or primary/replica control. The peqSTAR 96X even offers an emulation mode function: Selecting another cycler model from the drop-down menu will...

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PCR solutions from Avantor-9

Which VWR PCR cycler fits best for you? All made to provide excellent PCR results in shortest times and offering the simplest operation the Avantor range of VWR thermal cyclers is targeting different needs. Check out the below table that will help to make a smart choice. Budget saving Block format Gradient option FlexGradient, Emulation mode Free PC software include user management and MP3 capablilty High pressure lid (HPL) option Maximum ramp rate Solutions for discovery VWR® Gel Documentation & Western blot Imaging A wide range of ins

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PCR solutions from Avantor-10

CYCLERS THERMAL CYCLERS XT96 AND XTENDER96 The XT family of VWR PCR thermal cyclers combines industry- and customerinspired innovation housed in a robust, low noise design that fits on virtually any bench top. The fast ramping 96-well block is accurately controlled by eight Peltier elements ensuring reproducible PCR results and offering a gradient PCR option. Making use of advanced technology, XT cyclers can be connected via Ethernet and controlled remotely by a primary XT96 cycler, a PC or a mobile device (smartphone/tablet). The software/app needed comes for free and provides the same intuitive...

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PCR solutions from Avantor-11

PCR thermal cyclers All made to provide excellent PCR results in shortest times and offering the simplest operation. Easy to find at vwr.com. Quick search by selector PCR Solutions from Avantor | vwr.com

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PCR solutions from Avantor-12

THERMAL CYCLERS The VWR® thermal cycler family combines high quality engineering with a comprehensive range of block formats. The UNO cycler is designed around a powerful, yet easy to use, software interface, and uses the same platform for both 96-well and 384-well formats. The Doppio, with two independent high speed 48well blocks in one system, offers the optimum solution for maximum flexibility within a minimum footprint. The Ristretto is a compact personal cycler with the highest flexibility, having a universal block that can be loaded with either 32×0,2 ml tubes or 16×0,5 ml tubes with flat...

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*Prices are pre-tax. They exclude delivery charges and customs duties and do not include additional charges for installation or activation options. Prices are indicative only and may vary by country, with changes to the cost of raw materials and exchange rates.