CSH130 C18 Columns for Peptide Separations Brochure
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CSH130 C18 Columns for Peptide Separations Charged Surface Hybrid particles deliver superior peptide separations in LC or LC/MS applications Waters patented synthesis process for its Charged Surface Hybrid (CSH™ Technology) particles imparts a low level positive charge to the surface of each particle. CSH Technology allows the columns to be successfully used with standard TFA-containing eluents or with a weaker acid modifier such as formic acid. This means users no longer have to compromise between selecting a reversed-phase eluent that delivers needed sharp and symmetrical separated peaks (e.g., 0.1% trifluoroacetic acid [TFA]) and one that minimizes reduction of MS signal (e.g., 0.1% formic acid [FA]). In similar fashion, the ability of the CSH130 C18 chemistry to accept greater peptide mass loads than on many other columns will enhance the ability to detect potentially important low level constituents contained with the major component(s) of interest. ■ Outstanding peak capacities in HPLC, UPLC®, or LC/MS separations of peptide and small proteins up to approximately 10,000 Daltons using either 0.1% FA- or 0.1% TFA-containing eluents. Excellent mass loading of complex peptide samples for peptide mapping, proteomics applications, or for the analysis and lab-scale purification of synthetic peptides. Scalable chemistries from nano flow analysis to lab-scale purification. QC tested with a cytochrome c tryptic digest using 0.1% FA-containing eluents to help ensure column-to-column reproducibility for consistent performance in validated methods.

Waters CSH130 C18 particles contain a low and carefully-defined concentration of positive charges that yield comparatively excellent peak shape for peptides using either FA- or TFA-containing mobile phases. The fact that the performance of a CSH130 C18 column exhibits little dependence on strong ion pairing agents makes it ideal for LC or LC-MS applications. Comparative Averaged Peptide Peak Capacities on Selected Reversed-Phase Columns with Differing Concentrations of FA and TFA Peak Capacity 2 Fold Decrease in MS Peak Area Effect of TFA on peak capacity and MS signal. (A) Peak capacity as...

SCALABL E COLUMN OFF ERINGS FOR H P LC- O R U P LC-BASED SEPA RAT IONS There remains a need for columns that are compatible with HPLC instrumentation. Low dispersion LC instrumentation is recommended in order to get full performance from a well-packed column containing 1.7 μm particles. The recent introduction of Waters eXtended Performance [XP] columns packed with 2.5 µm particles has allowed scientists to improve their existing HPLC instrumentation productivity. The high peak capacity peptide separations obtained with a CSH130 C18, 1.7 µm column could, for instance, be successfully scaled...

ORDERING INFO RMAT ION ACQUITY UPLC and XSelect CSH130 C18 Analytical Columns Kit Part Number(a) Part Number ACQUITY UPLC CSH130 C18, 1.0 x 50 mm, 1.7 µm Column ACQUITY UPLC CSH130 C18, 1.0 x 100 mm, 1.7 µm Column ACQUITY UPLC CSH130 C18, 1.0 x 150 mm, 1.7 µm Column ACQUITY UPLC CSH130 C18, 2.1 x 50 mm, 1.7 µm Column ACQUITY UPLC CSH130 C18, 2.1 x 100 mm, 1.7 µm Column ACQUITY UPLC CSH130 C18, 2.1 x 150 mm, 1.7 µm Column ACQUITY UPLC CSH130 C18 2.1 x 50 mm, 1.7 µm VanGuard™ Column, 3/pk ACQUITY UPLC CSH130 C18, 2.1 x 150 mm, 1.7 µm Column Method Validation Kit (b) XSelect CSH130 C18, 2.1 x...

ORDERING INFORMATION (e) For use in developing lab-scale preparative chromatography ® Requires 10 x 10 mm Cartridge Holder, Part No. 289000779 ® Requires 19 x 10 mm Cartridge Holder, Part No. 186000709 1) "Increasing Peak Capacity in Reversed Phase Peptide Separations with Charged Surface Hybrid (CSH) C18 Columns" M.A. Lauber, S.M. Koza, K.J. Fountain Waters Application Note, 720004568EN, 2013 2) "Peptide Mapping and Small Protein Separations with Charged Surface Hybrid (CSH) C18 and TFA-Free Mobile Phases" M.A. Lauber, S.M. Koza, K.J. Fountain Waters Application Note, 720004571EN, 2013 3)...