[ HPLC COLUMNS ] HPLC Column Performance

HPLC Columns Creating Exceptional Chromatography Waters® reputation is based on chromatography, but we do not create chromatography — you do. Innovative thinking within your laboratory creates the chromatographic methods and assays that sustain your business. The metric of your success is monitored by the methods and results that you produce, and the HPLC column that you choose today needs to support your success for the future. Waters full line of state-of-the-art, reversed-phase and HILIC HPLC columns are chosen by scientists who understand that performance and innovation are linked and their...

CORTECS XBridge XSelect Atlantis SunFire Symmetry XTerra Waters Spherisorb Nova-Pak Resolve Delta-Pak µBondaPak/BondaPak µPorasil/Porasil VanGuard Waters Analytical Standards and Reagents

Solid-core particle packing materials combine a fully-porous surface layer that has been bonded to a solid-core substrate. This combination creates a highly efficient particle substrate that maintains chromatographic resolution while offering the advantage of lower column back pressures. CORTECS® 2.7 pm Solid-Core Particle Columns maximize resolution and peak capacity for all LC separations and are optimized to extend HPLC or UHPLC instrument performance. The innovative solid-core technology and bonding chemistry used in CORTECS Columns helps you by: ■ Reducing Operational Backpressure: Lower...

Reduced Backpressure CORTECS Columns reduce operational backpressure allowing you to run methods using conventional LC instrumentation without compromising efficiency or resolution. Additionally, longer columns can be used to improve resolution for co-eluting peaks in complex sample mixtures. Backpressure Advantages of CORTECS 2.7 µm Columns Flow Rate Efficiency Advantages of CORTECS 2.7 µm Columns Flow Rate Comparative separations may not be representative in all applications. Comparative separations may not be representative in all applications. CORTECS 2.7 µm Columns offer a 25% reduction...

A dedicated selection of 7 phases can be used to separate a wide array of compound classes. CORTECS C18 provides a balanced retention profile for acidic, basic, and neutral compounds. CORTECS C18+ gives the best peak shape and increased sensitivity of basic analytes when using low ionic strength mobile phases such as formic acid. CORTECS T3 is an excellent phase to use when separating compounds of various polarity. The lower C18 ligand density provides balance retention for both polar and nonpolar compounds and the 120A pore diameter allows for the use of 100% aqueous mobile phase. CORTECS C8,...

Based on BEH Technology Ethylene Bridged Hybrid (BEH) Technology synthesis creates particles that ensure extreme column performance and long column lifetimes under harsh operating conditions. T he particle is prepared from two high purity monomers: tetraethoxysilane (TEOS) and bis(triethoxysilyl) ethane (BTEE), which results in a highly stable, pH resistant, and mechanically strong particle. XBridge ® BEH HPLC Columns are designed for one purpose, to maximize your productivity. Whether your goal is to create a quality control method or develop a leading edge LC-MS assay, XBridge BEH Columns are...

Much of the cost when developing a chromatographic method is associated with the rigorous testing and validation of the final method. We understand that revalidation of your method is not an option, so we thoroughly test each batch of sorbent and final column product to ensure that you get the most reproducible columns available. With an XBridge BEH Column, you have the confidence that the method you develop today will be repeatable for the lifetime of your assay. XBridge Family USP Tailing Factors Particle Etticiency BEH Technology™ offers many advantages over conventional silica-based particles,...

Accelerated High pH Stability Test of Competitive Columns XBridge BEH Columns have been specifically designed to contain the most chemically-stable chromatographic sorbent available, allowing you to explore the full benefits of a wide pH (1–12) mobile-phase range. Chemical stability, especially for the extremes of pH, is built into the particle during the synthesis process and it cannot be duplicated using a conventional silica-based bonding process. No other column can match the chemical stability of an XBridge Column. Zorbax® Extend C18 Analyte: Chromatograms, run at regular intervals during...

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XSelect HPLC Columns are designed for the method development scientist who demands the most diverse selection of sorbents to easily separate the most difficult analyte co-elutions. XSelect Columns are tools that are: ■ Designed for Selectivity: Improve your ability to separate closely eluting peaks ■ Intended for Isolation and Purification: Highest analyte mass loading available ■ Ideal for Rapid Method Development: Reduce the time and cost spent developing methods The XSelect HPLC Column family features two base particles with a unique blend of optimized ligands to provide highly selective chromatographic...

Enhanced Selectivity Selectivity and retentivity are the most powerful tools a method developer has to influence chromatographic behavior. The XSelect family offers a diverse range of reversed-phase C18 columns (e.g., CSH C18, HSS C18, HSS C18 SB) for general purpose separations; as well as columns that offer improved polar retention (T3) and greater selectivity options (phenyl-hexyl, fluoro-phenyl, and cyano) for method development. XSelect Columns Provide Diverse Analyte Selectivity LC Conditions LC system: ACQUITY UPLC with ACQUITY UPLC PDA Detector Columns: 2.1 x 50 mm Mobile phase...

Method Development and Transfer When developing methods, skilled chromatographers realize that any method developed using uniquely selective columns must be easily transferable across laboratories, independent of the LC system platform used. XSelect Columns are engineered for method development and are fully compatible with all modern detection modes. Reproducible and Scalable Separations XSelect CSH Fluoro-Phenyl, 5 µm LC Conditions LC system: Columns: Flow rate: Mobile phase A: Mobile phase B: Gradient: Injection volume: Column temp.: Detection: ACQUITY UPLC with ACQUITY UPLC PDA Detector 2.1...