OASIS SOLID-PHASE EXTRACTION PRODUCTS
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[ OASIS SOLID-pHASEEXTRACTION PRODUCTS ] Solid-Phase Extraction Waters THE SCIENCE OF WHAT’5 POSSIBLE."

Through the combination of innovative sorbent technology and hardware design, Oasis® Products have become the first choice in solid-phase extraction (SPE). Oasis Products are trusted by separation scientists across the globe to meet a wide variety of sample preparation needs, ranging from a simple and fast matrix cleanup to the need to solve the most difficult and highly selective sample preparation challenges. Researchers rely on the superior technical performance of Oasis Products to achieve unmatched purity, consistency, and quality in their sample preparation methods.

[OASIS SOLID-pHASEEXTRACTION PRODUCTS ] ■ Fastest and simplest methods leading to the cleanest samples ■ Highest sensitivity for small molecules and therapeutic peptides ■ Reduced matrix effects for LC-MS/MS analyses ■ Unsurpassed selectivity ■ Highest SPE recovery ■ Exceptional product quality and reproducibility ■ Wide selection of sorbents ■ Broad array of formats [OASIS SOLID-pHASEEXTRACTION PRODUCTS ]

Oasis HLB Hydrophilic-Lipophilic Balanced Copolymer Simplifying Solid-Phase Extraction Waters introduced Oasis HLB in 1996, effectively changing the way scientists performed solid-phase extraction (SPE), as well as their expectations of this process. Constructed with a water-wettable copolymer that is stable from pH 0–14, Oasis HLB created a whole new range of solid-phase extraction method development possibilities. It is the gold standard in reversed-phase SPE, trusted by scientists across the world. Hydrophilic monomer Retention of polars Lipophilic monomer Reversed-phase retention...

[ OASIS SOLID-PHASE EXTRACTION PRODUCTS ] The ability to simplify and shorten SPE protocols is due to the unique water-wettable, balanced nature of the hydrophilic/lipophilic Oasis Sorbent. Silica-based sorbents and other “Oasis-like” polymeric sorbents that are not as water-wettable as Oasis HLB sacrifice performance when eliminating these condition and equilibration steps. The chart below illustrates the difference that a thoroughly water-wettable sorbent such as Oasis HLB can make in the implementation of this simplified, 3-step protocol. Oasis HLB is able to effectively retain a wide...

Oasis PRiME HLB Oasis PRiME HLB is the first-of-its-kind SPE sorbent that sets the new performance standard for routine analyses. The unique, patent-pending Oasis PRiME HLB Sorbent provides cleaner samples in less time and with less effort. These powerful and easy-to-use benefits are important since solid-phase extraction provides many benefits when performed prior to chromatographic analyses, especially when using mass detection. These benefits include a reduction in matrix effects and chromatographic complexity as well as the ability to increase sample concentration. However, scientists...

[ OASIS SOLID-PHASE EXTRACTION PRODUCTS ] Simpler Easy, efficient protocols Oasis PRiME HLB was created to have the same powerful benefits made possible with Oasis HLB. The Oasis PRiME HLB Copolymer is extremely water-wettable, making it possible to eliminate the condition and equilibration steps that are required when using silica-based or other polymeric sorbents. Without these additional steps, sorbents that are not as water-wettable are unable to effectively retain analytes of interest during the sample load step, causing a loss of recovery. The ability to skip these steps saves...

Faster, more even flows across cartridges and plates with less plugging Oasis PRiME HLB has been designed to increase speed both within the SPE device itself and in your laboratory's sample processing workflow. Not only can you eliminate the condition and equilibration steps, viscous samples flow more easily through the cartridges and 96-well plates due to a new, optimized frit and sorbent design. Flow rates through the devices are much faster for common matrices such as plasma, urine, and milk. The optimal load, wash, and elution flow rates are achieved using less vacuum or positive...

[ OASIS SOLID-PHASE EXTRACTION PRODUCTS ] Even Cleaner Oasis PRiME HLB removes more than 95% of common matrix interferences such as proteins, salts, and phospholipids, using the generic protocol without any additional optimization. Cleaner samples means less variability in your analytical results from sample to sample, increasing the robustness of your analyses. Phospholipids Remaining in Final Eluate Phospholipids Remaining in Final Eluate 7000000 6000000 5000000 4000000 3000000 2000000 1000000 0 Oasis PRiME HLB Fewer phospholipids remain in the final sample eluate with the Oasis PRiME HLB...

Sorbents The Mixed-Mode Ion Exchange/Reversed-Phase Sorbents are all based on the Oasis HLB Copolymer to provide a range of options for method development. Mixed-Mode Cation eXchange sorbent for bases Mixed-Mode Anion eXchange sorbent for acids Oasis HLB Hydrophilic-Lipophilic Balanced reversed-phase sorbent for acids, bases, and neutrals Hydrophilic Retention of Polars Oasis WCX Mixed-Mode Weak Cation eXchange sorbent for strong bases and quaternary amines Oasis WAX Mixed-Mode Weak Anion eXchange sorbent for strong acids Stable from pH 0–14 Water-wettable No silanol interactions Reduced...

[OASIS SOLID-pHASEEXTRACTION PRODUCTS ] The Oasis Mixed-Mode Sorbents, which are all 100~ built upon the unique water-wettable Oasis HLB Copolymer, provide both reversed-phase and ion exchange modes of retention, enabling greater cleanup selectivity and sensitivity for both acidic and/or basic compounds. An example of this 0 outstanding capability can be seen with the Oasis 1.54 pElution Plate. This product employs a patented 100 design to concentrate a sample up to 15x without requiring evaporation or reconstitution. LOQ of Alprazolam from Plasma B. 1 pg/mL spiked sample LC-MS/MS...

The increasing number of peptide based therapeutics brings new challenges to the typical bioanalytical laboratory. Sensitive, selective, and robust bioanalytical methods, which would ideally meet current regulatory criteria, must be developed for these types of compounds, which differ significantly from small molecule drugs. PROTOCOL Dilute plasma with 4% H3PO4 Because of their size and charge state distribution, sensitivity by mass spectrometry may be lower for biomolecules than typical small molecules, necessitating sample concentration and other means of increasing overall method...