OASIS SOLID-PHASE EXTRACTION PRODUCTS
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[ DISQUE DISPERSIVE SAMPLE PREPARATION EXTRACTION PRODUCTS ]

Dispersive Sample Preparation The QuEChERS method (an acronym for Quick, Easy, Cheap, Effective, Rugged, and Safe) followed by dispersive solid-phase extraction (d-SPE), is a simple and straightforward sample preparation technique ideal for multi-residue analysis for pesticides, veterinary drugs, and mycotoxins in a wide variety of food and agricultural products. DisQuE Dispersive Sample Preparation Extraction Products are conveniently packaged with pre-weighed sorbents and buffers in pouches and tubes as described in regulatory methods and protocols.

[ QuEChERS SIMPLIFIED ] The Science Behind DisQuE To overcome the deficiencies of traditional methods, an improved extraction procedure was developed (Anastas­ iades et al.)* s to extract pesticides from fruits and vegetables. The procedure is based on an initial single-phase extraction using 10 or 15 g sample with acetonitrile at 1 mL acetonitrile per 1 g of sample. A liquid-liquid partition is created by adding excess salts and buffers to the extract. After centrifugation, the acetonitrile layer, containing the pesticide, is collected. The matrix can be further cleaned and the excess...

Simplified QuEChERS Method Sample Preparation Protocol Homogenize sample Transfer sample to 50 mL tube Add acetonitrile and internal standard - shake vigorously for 1 minute Add buffering salts and centrifuge Transfer supernatant to d-SPE cleanup tube 402 Pesticide Residues at 10 ppb ng/g In One 10 Minute Run Recovery Data for Three Types of Sample Matrices Fortified at 10 ng/g UPLC® Conditions LC system; Column; Part number; Column temp.; Sample temp.; Flow rate; Mobile phase A; Mobile phase B; Gradient; Waters ACQUITY UPLC® System ACQUITY UPLC BEH Ci8 1.7 pm, 2.1 x 100 mm 186002352 40...

[ QuEChERS SIMPLIFIED ] Selection of d-SPE Product for Sample Cleanup Commodity Type Cleanup Provided Removes polar organic acids, some sugars and lipids Vegetables (Celery, Head Lettuce, Melon) Fruits and Vegetables with Fats and Waxes (Cereals, Nuts, Dairy, Avocado) Removes polar organic acids, some sugars, more lipids and sterols Cleaning Up Pigmented Fruits and Vegetables Some fruits and vegetables, such as spinach, red sweet pepper, and carrots, have high content of non-polar pigments, such as carotenoids or chlorophyll. QuEChERS effectively removes some common matrix constituents,...

Ordering Information Kits Name Pouch: 1.5 g sodium acetate and 6 g MgSO 4 50 mL Tube: Empty 2 mL Tube: 150 mg MgSO 4 and 50 mg PSA Pouch: 1 g trisodium citrate dihydrate, 0.5 g disodium hydrogencitrate sesquihydrate, 1 NaCl and 4 g MgSO 4 50 mL Tube: Empty 2 mL Tube: 150 mg MgSO 4, 25 mg PSA, and 25 mg C18 DisQuE Dispersive SPE Kit (AOAC Method 2007.01) 50 mL Tube: 1.5 g sodium acetate, 6 g MgSO 4 2 mL Tube: 150 mg MgSO 4, 50 mg PSA DisQuE Dispersive SPE Kit (CEN Method 15662) 50 mL Tube: 4 g MgSO 4, 1 g NaCl, 1 g trisodium citrate dehydrate, 0.5 g disodium hydrogencitrate sesquihydrate 2...

2 mL Tube-White Cap 2 mL Tube-Blue Cap 2 mL Tube-Yellow Cap 2 mL Tube-Green Cap 2 mL Tube-Blue Cap 2 mL Tube-Yellow Cap 2 mL Tube-Blue Cap 2 mL Tube-Blue Cap 15 mL Tube-White Cap 15 mL Tube-Blue Cap 15 mL Tube-Clear Cap 15 mL Tube-Blue Cap 15 mL Tube-Blue Cap 15 mL Tube-Clear Cap 15 mL Tube-Blue Cap 15 mL Tube-Blue Cap 15 mL Tube-Blue Cap Al-N = Alumina N

sales offices: Waters THE SCIENCE OF WHAT’S POSSIBLE.® Waters, The Science of What's Possible, UPLC, ACQUITY, and ACQUITY UPLC are registered trademarks of Waters Corporation. DisQuE is a trademark of Waters Corporation. All other trademarks are the property of their respective owners. ©2015 Waters Corporation. Printed in the U.S.A. July|2016 720003048E|JaW-SIgI