Oasis µElution Plate Brochure
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[ product solution ] Ideal for small sample volumes Pat ent ed Innovat ion Now you can confidently perform SPE cleanup and analyte enrich- Eluting in 25 µL without evaporation provides up to a 15–fold ment of very small sample volumes (10-25 µL) up to a maximum of increase in analyte concentration, enabling sensitive, robust, and 375 µL. The Waters Oasis µElution plate combines patented plate reproducible SPE results. Scientists in both pharmaceutical drug design, proven Oasis chemistries , and straightforward protocols that discovery and drug development can prepare the cleanest biological deliver high analyte recovery and clean extracts in elution volumes as sample extracts for more sensitive LC/MS/MS analysis. low as 25 μL. Using the Oasis μElution plate achieves superior results compared to other conventional SPE formats in less time. This plate format produces concentrated extracts that can be directly injected into your LC/MS/MS, eliminating the need for the time-consuming evaporation step.

Rapid and Sensitive Method for the Determination of Ropinirole in Human Plasma Ropinirole is a potent drug marketed under the name Requip® in the U.S. and Adartrel® in Europe. It is prescribed for both restless leg syndrome and Parkinson’s disease. A bioanalytical method was developed and validated using Oasis MCX μElution plates for sample preparation and concentration, and an ACQUITY UPLC®/MS/MS for highest sensitivity. The required LLOQ (lower limit of quantitation) of 0.005 ng/mL (5 pg/mL) for ropinirole in human plasma was easily achieved. The response at 0.005 ng/mL is more than 10x...

[ product solution ] Sample Enrichment: Up to a 15X Concent ration Factor The Oasis 2x4 method is a simple, logical approach to the selection of 0.5 ng/mL risperidone 375 µL sample diluted with 375 µL 4% H3PO4 loaded onto Oasis MCX plates Elution in 25 µL Recovery = 98% 11X increase in S:N 17X increase in area counts Oasis MCX µElution plate 15X concentration recoveries while removing matrix components that may interfere with This flow chart outlines the simple steps required to achieve high recovery and the cleanest extracts: • Characterize your analyte [neutral, acid or base; pKa]. •...

Oasis 2x4 Met hod Proof of Conc e pt Matrix Effects Study Recovery Study Matrix effects* are an alteration in MS response caused by interfer- To demonstrate the logic, simplicity, and effectiveness of the Oasis 2x4 method, five samples of rat plasma were prepared, each spiked with one of the previously characterized test analytes shown below: P roof of Concept: Analytes Spiked into Rat Pl asma ing components in a sample. They may cause a loss of signal, ion suppression or gain in signal, ion enhancement. Phospholipids or lysophopholipids have been identified and reported as a major...

μElut ion P lat e Loading Ca pacit y SPE device capacity is defined as the total mass of analytes and SPE Recovery for 200 ng/mL Imipramine and 200 ng/mL Atenolol on Oasis MCX µElution Plate endogenous sample components retained by the sorbent bed under SPE Recovery for Polar and Non-Polar Analytes in Plasma Example loading conditions. Breakthrough will occur when the capacity of the sorbent bed is exceeded. The physicochemical properties of Oasis sorbents are designed to provide exceptionally high loading capacity, 2 mg of Oasis sorbent. even though each well in a Waters Oasis µElution...

Oasis 96-w ell µElut ion P lat es (1/pkg) Description Part Number Oasis Sorbent Selection Plate, 3 rows each MCX, WAX, MAX and WCX Oasis Elution Plate Extraction plate manifold for Oasis 96-well plates Part Number Extraction plate manifold kit A (includes extraction plate manifold, reservoir tray, sealing cap and 350 µL sample collection plate) Disposable reservoir tray Sample collection plate, 350 µL Sealing cap for 96-well collection plate SPE Vacuum Pump (Includes two gauges and pressure regulator) Vacuum box gasket kit Kit includes: 2 foam top gaskets 2 orange O-rings Waters, The...