productivity comes from

INCREASE YOUR PRODUCTIVITY THROUGH HIGHER RECOVERIES AND LONGER COLUMN LIFETIMES With Optimum Bed Density (OBD™) Preparative Columns you have the ability to: ■ Achieve fast, efficient, lab-scale separations for greater throughput Directly scale from UPLC,™ UHPLC, or HPLC screening to lab-scale purification Select robust chromatographic particles designed for purif

HOW TO CHOOSE THE RIGHT OBD PREPARATIVE COLUMN STEP 1 Once the analytical separation has been optimized, a loading study on the analytical column is performed to determine the capacity of the particular packing material. The large scale separation should be identical to the small scale separation, therefore the maximum sample load will be dependent upon the complexity of the analytical separation. STEP 2 Determine how much mass you need to purify or isolate. STEP 3 Use these simple equations to determine the required column size for purification. Note: Preparative HPLC system maximum flow rate...

Why struggle with inconsistencies in column-to-column performance, diminished column lifetimes, lost samples, repeat purification runs, and poor scalability from small to large volume columns? THE OBD COLUMN DESIGN Conventional Prep Column Packing Before Optimum Bed Density (OBD) Column Packing* Packing Unstable region Flow (no voids) Compress The patented OBD Preparative Column design and packing process results in predictable, uniform density profiles throughout the column. During the final capping process, our established procedures do not over compress or disrupt, in any nonuniform way, this...

COLUMN STABILITY AND RELIABILITY — LONG, PREDICTABLE LIFETIMES The demand for rapid, high-purity, compound isolation places strong emphasis on the integrity and stability of preparative columns. Complex, sparingly-soluble starting materials are often dissolved with strong solvents, such as DMSO. The combination of poor solubility and pressure shocks associated with large injection volumes of pure organic solvent are the primary contributors to early column failure and chromatographic bed collapse. The OBD design exhibits exceptional resistance to mechanical chromatographic bed failure and delivers...

SCALABILITY – BRIDGING THE GAP FROM ANALYTICAL TO PREPARATIVE CHROMATOGRAPHY Successful scaling of methods from analytical to preparative dimensions requires the use of chromatographically equivalent columns. All too often, even when the same chemistry phase and particle size are used, methods do not scale either due to loss of resolution and/or lower than expected loading. By matching the analytical and preparative column bed densities, scalability is assured, eliminating the need for any time consuming method redevelopment. OBD Preparative Columns are packed to bed densities which closely match...

MASS LOADING Many factors affect the mass capacity of preparative columns. The listed capacities represent an “average” estimate. Capacity is: ■ Higher for strongly retained material Higher for simple mixtures Lower where higher resolution is required Very strongly dependent on loading conditions Limited by loading volume Approximate mass loading capacities (mg) for OBD Preparative Columns (Gradient mode) Limited by diluent solvent strength Reasonable flow rate (mL/min) Reasonable injection volume (µL) Reasonable flow rates are based on column diameter. Systems will be limited by increasing backpressure...

[ PREPARATIVE OBD COLUMNS ORDERING INFORMATION ] XSelect Preparative Columns Dimension Particle size Particle size Requires 10 x 10 mm Prep Guard

XBridge Preparative Columns Dimension Particle size

[ PREPARATIVE OBD COLUMNS ORDERING INFORMATION ] SunFire Preparative Columns Atlantis Preparative Columns Particle size Particle size Requires 10 x 10 mm Prep Guard Holder, p/n: 289000779 2 Requires 19 x 10 mm Prep Guard Holder, p/n: 186008745 3 Requires 30 x 10 mm Prep Guard Holder, p/n: 186006912

[ PREPARATIVE OBD COLUMNS ORDERING INFORMATION ] Protein Preparative Columns Peptide Preparative Columns Dimension Particle size Requires 10 x 10 mm Prep Guard Holder, p/n: 289000779 Requires 19 x 10 mm Prep Guard Holder, p/n: 186008745 3 Requires 30 x 10 mm Prep Guard Holder, p/n: 186006912 1 Literature References White Paper BEH (Ethylene Bridged Hybrid) Technology CSH (Charged Surface Hybrid) Technology OBD (Optimum Bed Density) Technology Requires 10 x 10 mm Prep Guard Holder, p/n: 289000779 2 Requires 19 x 10 mm Prep Guard Holder, p/n: 186008745 3 Requires 30 x 10 mm Prep Guard Holder, p/n:...

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