Torus™
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Torus Columns a. Safety Considerations b. Column Connectors and Installation c. Column Equilibration d. eCord Installation e. Initial Column Efficiency Determination f. VanGuard Pre-Columns a. Sample Preparation b. Solvents c. Additives d. Pressure e. Temperature f. Useable Flow Rate Ranges and Expected ACQUITY UPC 2 System Pressures g. Band Spreading Minimization IV. T ROUBL E SHOOT ING V. COLUMN C L EANING, A DDIT IV E S, AND STO RAG E a. Cleaning b. Effects of Additives c. Aging d. Storage a. Introduction b. Installation c. Column Use Information I. INT RODUC T ION Thank you for choosing a Torus™ Column. Torus Columns are offered with 1.7 µm and 5 µm particles, allowing scientists to transfer SFC achiral separations from analytical to preparative scale. Torus 1.7 µm Columns are specifically designed to use the complete range of capabilities offered by the ACQUITY UPC 2 System to achieve fast, robust achiral separations. Torus Columns simplify the method development process with four completely new and innovative 1.7 μm chemistries for UltraPerformance Convergence Chromatography™. These columns are designed for excellent peak shape, coupled with added selectivity for a wide range of compounds, and improved robustness. Torus 5 µm Columns are offered in both analytical and preparative column formats. The Torus OBD™ Preparative Columns combine the patented* optimized packing process with a unique set of column designs ensuring increased lifetime and greater reproducibility. These columns are based on new bonding technology used to create an adaptive selectivity platform. Starting with the ethylene bridged hybrid (BEH) particle, the patent pending bonding consists of a two stage functionalization of chromatographic particles. The first stage controls retention characteristics and is applied to each sorbent. The individual second stage selectors impart the observed selectivity and control peak shape. (* US Patent # 7,399,410, UK Patent # GB2

Achiral chemistry Stationary phase Particle shape Torus DIOL High-density Diol Table 1. Physical Characteristics. The Torus packing materials are designed for use with the ACQUITY UPC 2 Systems and for the Waters SFC Purification Systems. Torus Columns are manufactured in an ISO-certified plant using ultra-pure reagents. Each batch of Torus particles are tested and the results are held to narrow specification ranges to assure excellent, reproducible performance. Every column is individually tested and a Performance Chromatogram and Certificate of Batch Analysis is provided . For Torus 1.7...

c. Column Equilibration Torus Columns are shipped dry. Equilibrate the column with a minimum of 10-column volumes of the mobile phase prior to use. (Refer to Table 2 for a listing of empty column volumes.) Thermal and chemical equilibration are equally important for chromatography. Ensure that the mobile phase is preheated before entering the column to prevent a thermal mismatch. This can be done by enabling active preheating on an ACQUITY UPC2 instrument. Empty column volumes (mL) Column internal diameter (mm) Column length Table 2. Empty column volumes in mL (multiply by 10 for flush...

4. From below, insert VanGuard Pre-column into Torus 1.7 µm Column inlet and hand-tighten (collet and ferrule are not yet permanently attached). 5. While pushing the VanGuard Pre-column into the column inlet, turn assembled column and pre-column 180˚ so that precolumn is now on top. 6. Tighten with two 5/16” wrenches placed onto Torus Column flats and VanGuard Pre-column hex nut (male end) as shown above. 7. Tighten 1/4 turn to set collet and ferrule. 8. Check that ferrule is set by loosening connection and inspecting the ferrule depth. A properly set ferrule depth will resemble other...

Predicted max flow rate (mL/min) Predicted max flow rate (mL/min) Table 3. Useable flow rate ranges and expected ACQUITY UPC2 System pressures with Torus 1.7 µm Columns. e. Temperature The general guideline for maximum temperature for Torus Columns is 60° C with most common SFC mobile phases and additives. However, some data shows good stability beyond 60° C. This would need to be verified experimentally. f. Useable Flow-Rate Ranges and Expected ACQUITY UPC 2 System Pressures Table 3 estimates maximum flow rate and system pressure which can be achieved without over-pressuring the ACQUIT Y...

V. COLUMN C L EANING, EFF EC T S OF ADDIT IV ES, AGING, AND STORAGE Changes in peak shape, peak splitting, shoulders on the peak, shifts in retention, change in resolution, or increasing backpressure may indicate contamination of the column. Flushing with high concentrations of organic solvent, taking care not to precipitate buffers, is usually sufficient to remove the contaminant. If the flushing procedure does not solve the problem, purge the column using the following cleaning and regeneration procedures. a. Cleaning Use the cleaning routine that matches the properties of the samples...

V I. INT RODUC ING ECO RD INT E L LIG ENT C HI P T EC HNOLOGY a. Introduction Supplied with the Torus 1.7 µm Columns, the eCord Intelligent Chip will provide the history of a column’s performance throughout its lifetime. The eCord is permanently attached to the column to assure that the column’s performance history is maintained in the event that the column is moved from one instrument to another. Figure 4. Installing the eCord Intelligent Chip. Figure 3. eCord Intelligent Chip. At the time of manufacture, tracking and quality control information will be downloaded to the eCord. Storing...