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Waters Sep-Pak Sample Extraction Products Brochure

Waters Sep-Pak Sample Extraction Products Brochure
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Waters Sep-Pak Sample Extraction Products Brochure

Product catalog summary
Introduction
Sep-Pak Cartridges are essential in solid-phase extraction (SPE) techniques, widely recognized for their reliability in sample preparation across various scientific fields, as referenced by organizations like the U.S. EPA and AOAC International.
Product Specifications
Available in multiple formats and sorbents such as C18, tC18, C8, tC2, Silica, and Florisil, Sep-Pak Cartridges are designed with specific mass/volume ratios. They are made from high-density polyethylene for solvent resistance and tested for low UV-absorbing extractables.
Benefits of Sep-Pak Cartridges
These cartridges enhance sample preparation by reducing costs, increasing recovery rates, simplifying protocols, improving safety, and accuracy. They minimize sample handling and the need for transporting samples to labs, preserving labile samples and reducing glassware usage.
Solid-Phase Extraction (SPE) Process
SPE involves introducing a sample into a cartridge with chromatographic packing material, selecting appropriate mobile and stationary phases to either pass components through or retain them for analysis.
Anatomy of a Sep-Pak Cartridge
Featuring high-quality packings and radial compression technology, Sep-Pak Cartridges are designed for manual and robotic applications, sealed in non-contaminating pouches to maintain integrity.
Chromatography Techniques
Supporting both normal-phase and reversed-phase chromatography, Sep-Pak Cartridges are used for separating neutral organic compounds and a broader range of analytes, respectively.
Conclusion
With over 200 combinations of sorbents and device formats, Sep-Pak products offer versatile solutions for analytical challenges, ensuring simplicity, selectivity, and speed in sample preparation.
Introduction to Solid-Phase Extraction (SPE)
SPE is used for isolating specific compounds from mixtures, applied in fields like environmental analysis, food and beverage testing, and biological fluid analysis.
Reversed-Phase Chromatography
Predominant in SPE for aqueous samples, it uses a gradient of solvents with non-polar packing material, concentrating dyes near the inlet of the SPE cartridge.
Ion-Exchange Chromatography
Ideal for ionic compounds, it uses a gradient of pH or ionic strength with ion exchange packing material, forming bands near the inlet. Mixed-mode may involve an organic solvent wash to elute neutral compounds.
Sep-Pak Device Formats
  • Sep-Pak Plus Cartridges: Versatile for robotic and manual applications.
  • Sep-Pak Light Cartridges: Reduced volume for limited samples, improving sensitivity.
  • Sep-Pak Vac RC Cartridges: Larger capacity for greater volumes, reducing setup time.
  • Sep-Pak Classic Cartridges: Original design allowing method transfer to Plus cartridges.
  • Sep-Pak Vac Cartridges: Economical for automation with various sizes.
  • Sep-Pak 96-Well Extraction Plates: High throughput automation compatible.
  • Sep-Pak 96-Well µElution Plates: For small volumes, increasing analyte concentrations.
Product Information and Ordering
Sep-Pak products, including cartridges and plates, are available with different sorbents and configurations. Detailed information and ordering options are accessible through Waters Corporation's website.
Contact Information
Waters Corporation provides global contact details for sales offices, ensuring customer accessibility worldwide.
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Catalog excerpts

Waters Sep-Pak Sample Extraction Products Brochure-1

[ SEP-PAK SAMPLE EXTRACTION PRODUCTS ] THE ORIGINAL AND MOST REFERENCED SILICA-BASED SPE PRODUCTS

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Waters Sep-Pak Sample Extraction Products Brochure-2

[ SEP-PAK SOLID-PHASE EXTRACTION PRODUCTS ] SAMPLE EXTRACTION PRODUCTS ] [ SEP-PAK C ARTRIDGES ] Pioneering a Revolution in Modern Sample Preparation Sep-Pak® Cartridges catalyzed the dramatic growth of an industry devoted to solid-phase extraction (SPE). After 35 years of success in streamlining sample preparation, Sep-Pak Cartridges continue to be the most widely referenced SPE products in the world [by more than a nine-fold margin, > 137,000 articles!*]. Sep-Pak Cartridges are specified in many official methods including those published by the U.S. EPA, AOAC International, ASTM, NIOSH, CDFA,...

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Waters Sep-Pak Sample Extraction Products Brochure-3

[ SEP-PAK SOLID-PHASE EXTRACTION PRODUCTS ]] [ SEP-PAK SAMPLE EXTRACTION PRODUCTS

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Waters Sep-Pak Sample Extraction Products Brochure-4

[ SEP-PAK SOLID-PHASE EXTRACTION PRODUCTS ] SPE IS LIQUID CHROMATOGRAPHY Sep-Pak Cartridges use the code of liquid chromatography and a broad polarity spectrum of sorbents—including silica, acidic, neutral, and basic aluminas, Florisil™, and a selection of bonded silicas—to: Derivatize analytes using solid-phase reagents. Fractionate classes of compounds. Remove sample constituents that elute after the analytes of interest, or are strongly adsorbed on, LC columns. Remove sample constituents that coelute with an analyte of interest on LC columns. Desalt samples. Exchange solvents. Enrich sample...

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Waters Sep-Pak Sample Extraction Products Brochure-5

ANATOMY OF A SEP-PAK CARTRIDGE [ Sep-Pak Classic ] A Precision Chromatographic Tool for Sample Enrichment and Purification Here are ten unique features that have set Sep-Pak Classic Cartridges apart from the competition and made them the most widely-referenced column liquid-solid extraction cartridges available today. Flexible inlet accepts male Luer tip for positive seal under pressure. Seamless body of virgin irradiation-crosslinked polyethylene has excellent solvent, stress-crack, and temperature extreme resistance. Tubing is specially selected and quality tested for very low level of UV-absorbing...

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Waters Sep-Pak Sample Extraction Products Brochure-6

[ SEP-PAK SOLID-PHASE EXTRACTION PRODUCTS ]] [ SEP-PAK SAMPLE EXTRACTION PRODUCTS [ Sep-Pak Plus ] The Standard Among Devices for Sample Enrichment and Purification Here are twelve unique features that make Sep-Pak Plus Cartridges the finest column liquid-solid extraction cartridges in the world. Female inlet accepts male Luer tip; enables cartridges to be stacked for increased capacity or for multidimensional separations with different column chemistries. Injection-molded body of virgin high-density polyethylene has excellent solvent resistance. All body parts are quality tested to verify very...

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Waters Sep-Pak Sample Extraction Products Brochure-7

[ SEP-PAK SOLID-PHASE EXTRACTION PRODUCTS ] SAMPLE EXTRACTION PRODUCTS ] SIMPLICITY, SELECTIVITY, SPEED.

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Waters Sep-Pak Sample Extraction Products Brochure-8

[ SEP-PAK SAMPLE EXTRACTION PRODUCTS ] [ SEP-PAK SOLID-PHASE EXTRACTION PRODUCTS ] No matter what your analyte of interest or sample type [chemical, environmental, food, or biological matrix], the wide variety of Sep-Pak sorbents and device formats [more than 200 combinations] and tens of thousands of published procedures make it very likely that you will find a Sep-Pak sample extraction product to meet your analytical challenge. General Elution Protocols There are two general strategies for isolating and cleaning up sample components of interest: 1. Adsorb matrix interferences while components...

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Waters Sep-Pak Sample Extraction Products Brochure-9

[ SEP-PAK SOLID-PHASE EXTRACTION PRODUCTS ]] [ SEP-PAK SAMPLE EXTRACTION PRODUCTS Reversed-Phase Chromatography Because of the multiplicity of aqueous samples spanning a breadth of applications from environmental water to fruits and vegetables, from beverages to biological fluids, reversed-phase chromatography has become the predominant mode of SPE. To perform reversed-phase chromatography with SPE cartridges, use a gradient of strongly to weakly polar solvents [from weak to strong solvent elution strength] with a non-polar packing material. This procedure is illustrated in the figure here for...

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Waters Sep-Pak Sample Extraction Products Brochure-10

[ SEP-PAK SOLID-PHASE EXTRACTION PRODUCTS ] SAMPLE EXTRACTION PRODUCTS ] SEP-PAK DEVICE FORMATS Sep-Pak Plus Cartridges: Maximum Versatility Plus cartridges feature a state-of-the-art packing process as well as a precision design, suited to robotic applications, that also makes manual use even more convenient. Certain sorbents are available in short-body, and others in longbody, configurations. Connections can be made to different types of devices such as filter units, positive-flow pumps and syringes, vacuum manifolds, some automated sample processors, and in-line reaction systems (e.g., for...

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Waters Sep-Pak Sample Extraction Products Brochure-11

[ SEP-PAK SOLID-PHASE EXTRACTION PRODUCTS ]] [[ SEP-PAK SAMPLE EXTRACTION PRODUCTS SEP-PAK SAMPLE Sep-Pak 96-Well Extraction Plates: High Throughput Automation Selected Waters Sep-Pak sorbents are available in a unique, multi-capacity, awardwinning 96-well plate format designed by Waters. Plates are molded from high-purity polypropylene. Wells are filled with the same high quality sorbents and frit materials available in the respective cartridge formats. Fluid capacity above the top frit in each well is approximately 2 mL. Thus, SPE protocols developed using Sep-Pak cartridges may be scaled down,...

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Waters Sep-Pak Sample Extraction Products Brochure-12

[ SEP-PAK SOLID-PHASE EXTRACTION PRODUCTS ]] [ SEP-PAK SAMPLE EXTRACTION PRODUCTS Sep-Pak Connections Kit Description Sep-Pak Connections Kit Sep-Pak Reservoir Part No. WAT011400 Additional Product Information Description Beginner’s Guide to SPE (Solid-Phase Extraction) Waters Sorbent Selection Guide for Solid-Phase Extraction Wall Chart Description Sep-Pak Reservoir, 30 cc www.waters.com/seppak Literature Code 715003405 720002007EN Search by literature code at waters.com

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Waters Sep-Pak Sample Extraction Products Brochure-13

[ SEP-PAK SOLID-PHASE EXTRACTION PRODUCTS ] SAMPLE EXTRACTION PRODUCTS ] SEP-PAK CARTRIDGE SELECTION GUIDE Plus Short Plus Long Plus Light Classic Short Classic Long Sep-Pak 96-Well Plates Description Sep-Pak DNPH-Silica Cartridge Part No. Description Sep-Pak DNPH-Silica Short Body Cartridge Sep-Pak DNPH-Silica Long Body Cartridge Sep-Pak Accell Plus QMA 100 mg Plate Sep-Pak XPoSure Aldehyde Sampler Cartridge Description XPoSure™ Aldehyde Sampler Cartridge Sep-Pak 96-Well µElution Plates Description Sep-Pak tC18 µElution Plate Sep-Pak Ozone Scrubber Description Ozone Scrubber

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*Prices are pre-tax. They exclude delivery charges and customs duties and do not include additional charges for installation or activation options. Prices are indicative only and may vary by country, with changes to the cost of raw materials and exchange rates.