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careGENE™ Zika Virus RT-PCR Kit

careGENE™ Zika Virus RT-PCR Kit

careGENE™ Zika Virus RT-PCR Kit

Product catalog summary
Introduction
The Zika virus is a single-stranded, positive-sense RNA virus from the Flaviviridae family, primarily transmitted to humans by Aedes mosquitoes. It has been endemic in Africa and Southeast Asia, with a significant outbreak starting in Brazil in April 2015.

careGENETM Zika Virus RT-PCR Kit Overview
The careGENETM Zika Virus RT-PCR kit is designed to meet public health needs by detecting the Zika virus with high sensitivity and specificity, as approved by the MFDS. It targets the Zika virus RNA encoding the envelope protein using a Taqman probe-based RT-PCR system.

Technology and Specifications
  • Detection Targets: Zika virus RNA target 1 (FAM), Zika virus RNA target 2 (CY5/Alexa647), Internal Control (VIC/HEX).
  • Instrumentation: Extraction with QIAamp Viral Mini Kit (QIAGEN); Amplification and Detection with ABI 7500, Bio-Rad CFX96.
  • Sample Type: Serum and urine.
  • Sample Storage: Below -20℃.
  • Input Volume: 5 uL RNA sample.
  • Limit of Detection: 0.1 PFU for both serum and urine.

Features and Benefits
  • Short turnaround time with pre-treatment within 3 hours.
  • Ease of use with reduced contamination risk due to single tube format.
  • Master mix format reduces hands-on time.
  • Excellent analytical accuracy and increased specificity through dual target detection.
  • Efficient quality control with endogenous internal control applicable to sample preparation.

Performance and Precision
  • Precision tests conducted with strong and weak positive serum samples showed consistent Ct ranges and low coefficient of variation (CV).
  • Dynamic range of 5-log using CDC samples (PRVABC59).

Clinical Performance
  • Tested on 200 human serum samples (50 positives, 150 negatives) and 165 urine samples (50 positives, 115 negatives) with 100% sensitivity and specificity.

Order Information
  • Product: careGENETM Zika Virus RT-PCR kit, 96 tests per kit, Catalog Number: MZK-N09682.

Components
  • 4 X 1 step RT-PCR Mix.
  • Zika primer/probe Mix.
  • Nuclease free water.
  • Aptima Zika Virus assay (Hologic) as a reference method.

Contact Information
WELLS BIO, INC., 16, Magokjungang 8-ro 1-gil, Gangseo-gu, Seoul, 07795, Republic of Korea. Tel: +82-2-3660-6900, Email: [email protected]
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Catalog excerpts

careGENE™ Zika Virus RT-PCR Kit-1

The Zika virus belongs to F/aviviridae, novel single-stranded, positive-sense RNA virus. Zika virus is believed to be transmitted to humans by infected Aedesspecies mosquitoes. Studies indicated that Zika virus has been endemic in Africa and Southeast Asia. The outbreak of Zika fever began in April 2015 in Brazil, and subsequently other countries in South America, Central America and the Caribbean.

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careGENE™ Zika Virus RT-PCR Kit-2

• Short turn around time Included pre-treatment within 3 hours Reduced contamination by single tube supplied • Master mix format Enables reduced hands-on time • Excellent analytical accuracy RT-PCR detection of Zika virus RNA in serum and urine specimens (patient-matched specimen) Through dual target detection Endogenous internal control can be applied sample preparation careGENE™ Zika Virus RT-PCR kit can detect Zika specific region encoding envelope protein using Taqman probe based RT-PCR system. The enzyme mixture contains reverse transcriptase and Taq DNA polymerase for the one step real...

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careGENE™ Zika Virus RT-PCR Kit-3

Strong positive Weak positive Ct range Mean CV Ct range Mean CV Dynamic range Amplication Plot

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careGENE™ Zika Virus RT-PCR Kit-4

• Aptima Zika Virus assay (Hologic) as a reference method • Samples from Boca Bolistics • Conventional Real-Time PCR System (Applied Biosystems 7500) The careGENE™ Zika Virus RT-PCR kit was tested to detect Zika infection using 200 human serum samples (50 positives and 150 negatives) by PECET in Columbia and KUMC Hospital in Korea. specimens valid - Result (%) tested tests Positive Negative The careGENE™ Zika Virus RT-PCR kit was tested to detect Zika infection using 165 human serum samples (50 positives and 115 negatives) by KUMC Hospital in Korea. specimens valid - Result (%) tested tests Positive...

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