15q22/6q21 Gene Abnormalities Probe Detection Kit

Product catalog summary

Product Overview
The 15q22/6q21 Gene Abnormalities Probe Detection Kit is designed for diagnostic use, utilizing fluorescence in situ hybridization (FISH) to detect gene abnormalities. It employs orange and green fluorescein to label the 6q21 and 15q22 probes, respectively.

Components and Storage
The kit includes a dual-color probe (100μL per tube) and should be stored below 0°C, ideally at -20°C±5°C, away from light. It is valid for 12 months, with limited freeze-thaw cycles.

Applicable Instruments
Fluorescence microscopy systems with specific filter sets for DAPI, Green, and Orange are required.

Sample Requirements
Bone marrow cell samples (1-3 mL) should be collected with heparin sodium anticoagulant. Fresh samples must be stored at 2-8°C for up to 24 hours, while fixed samples can be stored at -20±5°C for up to 12 months.

Testing Method

Reagents: Several reagents are needed, including SSC solutions, ethanol, NP-40/SSC solution, fixation solution, KCl solution, and DAPI counterstain.
Sample Pretreatment: Involves cell collection, washing, permeation, fixation, and preparation of cell suspensions.
Denaturation and Hybridization: Conducted in a darkroom, involves probe application, denaturation at 88°C, and hybridization at 45°C.
Washing: Includes steps in SSC and NP-40/SSC solutions.
Counterstaining: DAPI is used for staining, observed under a fluorescence microscope.

Signal Interpretation
Negative results show 2 Orange and 2 Green signals, while positive results show 3 of each.

Limitations and Precautions
Results may be affected by sample quality, hybridization conditions, and technical limitations. The kit is for in vitro diagnostic use only, and safety precautions must be observed due to potential toxicity of reagents.

Approval and Version
Manual version V1.0, approved on 01 September 2019.

Catalog excerpts

15q22/6q21 Gene Abnormalities Probe Detection Kit-1

Wuhan HealthCare Biotechnology Co., Ltd. Building #8, Optics Valley Precision Medicine Industry Base, #9 Gaokeyuan 3rd Road, East Lake High-Tech Zone. 430206, Wuhan, Hubei, China. Tel#: +86(027)8757-0662 Ext. #812 E-mail: [email protected] | Website: www.healthcare-biotech.com __________________________________________________________________________________________________ Product Catalogue Number FP-195 15q22/6q21 Gene Abnormalities Probe Detection Kit [Product Name] 15q22/6q21 Gene Abnormalities Probe Detection Kit (Fluorescence In Situ Hybridization Method). [Product Introduction] This kit uses Orange fluorescein to label the 6q21 orange probe, and Green fluorescein to label the 15q22 green probe. The 6q21/15q22 probe bind to the target detection site by in situ hybridization. [Product Main Components] The kit consists of 6q21/15q22 dual color probe as shown in Table 1. Component name 15q22/6q21 dual color probe Table 1 Kit composition Quantity 1 Main components 6q21 Orange probe ; 15q22 Green probe [Storage conditions & Validity] This kit is shipped below 0°C. Keep sealed away from light at -20oC± 5oC. The product is valid for 12 months. Avoid unnecessary repeated freezing and thawing that should not exceed 10 times. After opening, within 24 hours for short-term preservation, keep sealed at 2-8oC in dark. For long-term preservation after opening, keep the lid sealed at -20oC± 5oC away from light. [Applicable Instruments] Fluorescence microscopy imaging systems, including fluorescence microscopy and filter sets suitable for DAPI (367/452), Green (495/517), and Orange (547/565). [Sample Requirements] 1. Sample collection: Take 1-3 mL of heparin sodium anticoagulated bone marrow cell sample. 2. Sample storage: Unfixed fresh bone marrow cell specimen should be stored at 2-8°C for no more than 24 hours. After fixation, the cell suspension should be stored at -20±5°C for no more than 12 months. When the storage temperature of the specimen is too high or too low, or when the cell suspension is excessively volatile or contaminated during storage, the sample should not be used for testing. [Testing Method] 1. Related Reagents The following reagents are required for the experiment but not provided in this kit ① 20×SSC, pH 5.3±0.2 Weigh 176g of sodium chloride and 88g of sodium citrate, dissolve in 800mL of deionized water, adjust the pH to 5.3±0.2 at room temperature, and complete to 1 L with deionized water. High-pressure steam sterilization, stored at 2-8oC, the solution shelf life is of 6 months. Discard if the reagent appears cloudy (turbid) or contaminated. ② 2×SSC, pH 7.0±0.2 Take 100mL of the above 20xSSC, dilute with 800mL deionized water, mix, adjust the pH to 7.0±0.2 at room temperature, complete to 1L with deionized water, stored at 2-8oC, the shelf life is of 6 months. Discard if the reagent appears cloudy (turbid) or contaminated. _________________________________________________________________________________________________

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15q22/6q21 Gene Abnormalities Probe Detection Kit-2

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15q22/6q21 Gene Abnormalities Probe Detection Kit-3

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