CERTIFICATION AOAC Research Institute Performance Tested MethodsSM Certificate No. The AOAC Research Institute hereby certifies the method known as: Tianlong Biolum Portable ATP Hygiene Monitoring System manufactured by Xi’an Tianlong Science and Technology Co., Ltd. No.4266, Shanglin Road, Weiyang District Xi’an, 710021, Shaanxi P.R. China This method has been evaluated in the AOAC Research Institute Performance Tested MethodsSM Program and found to perform as stated in the applicability of the method. This certificate indicates an AOAC Research Institute Certification Mark License Agreement has been executed which authorizes the manufacturer to display the AOAC Research Institute Performance Tested Methods SM certification mark on the above-mentioned method for the period below. Renewal may be granted by the Expiration Date under the rules stated in the licensing agreement. Bradley A. Stawick, Senior Director Signature for AOAC Research Institute Issue Date Expiration Date 2275 Research Blvd., Ste. 300, Rockville, Maryland, USA Telephone: +1-301-924-7077 Fax: +1-301-924-7089 Internet e-mail: [email protected] * World Wide Web Site: http://www.aoac.org

SUBMITTING COMPANY Xi’an Tianlong Science and Technology Co., Ltd. No.4266, Shanglin Road, Weiyang District Xi’an, 710021, Shaanxi P.R. China METHOD NAME Tianlong Biolum Portable ATP Hygiene Monitoring System INDEPENDENT LABORATORY Q Laboratories 1930 Radcliff Drive Cincinnati, OH 45204 APPLICABILITY OF METHOD Target organism – Adenosine Triphosphate (ATP) Matrixes – Stainless steel (304) Performance claims – The Biolum Portable ATP Hygiene Monitoring System when used with the ATP Test Swab is effective at monitoring on-site cleanliness of stainless steel in food and beverage industries with...

Xi’an Tianlong Science and Technology Co., Ltd. Biolum ATP, AOAC Research Institute Performance Tested MethodsSM certification number 022401 Mean RLUa srb c RSD r Method Mean fmold developer sre RSDrf Mean RLU sr Independent RSDr Mean fmol laboratory sr RSDr a Average RLU from 10 replicates per ATP level. bs calculated from 10 replicates per ATP level. r c RSDr calculated from 10 replicates per RLU level. d Average ATP (femtomoles) from 10 theoretical replicates per ATP level. e s calculated from 10 predicted replicates per RLU level. r f s calculated from 10 predicted replicates per RLU level....

Xi’an Tianlong Science and Technology Co., Ltd. Biolum ATP, AOAC Research Institute Performance Tested MethodsSM certification number 022401 Figure 2. Regression Analysis used to Calculate LOD in Method Developer Study (1) Figure 3. Regression Analysis of RLUs to Determine ATP Concentration in Independent Laboratory Study (1)

Xi’an Tianlong Science and Technology Co., Ltd. Biolum ATP, AOAC Research Institute Performance Tested MethodsSM certification number 022401 Figure 4. Regression Analysis used to Calculate LOD in Independent Laboratory Study (1) Table 2. Raw and Calculated Data for the Stainless Steel Matrix Study – Wet Sampling (1) Dilutiona Mean RLUb

Xi’an Tianlong Science and Technology Co., Ltd. Biolum ATP, AOAC Research Institute Performance Tested MethodsSM certification number 022401 Mean RLUb

Xi’an Tianlong Science and Technology Co., Ltd. Biolum ATP, AOAC Research Institute Performance Tested MethodsSM certification number 022401 Table 7. Raw and Calculated Data for the Selectivity (1) RLU at 2500 fmol compound,0 fmol ATP NAb analyte-free water 1 ATP Adenosine 5'-triphosphate sodium salt hydrate 6720 dATP 2'-deoxyadenosine 5'-triphosphate sodium salt 431 UTP Uridine 5'-triphosphate trisodium salt 2 GTP Guanosine 5'-triphosphate sodium salt 1 TTP Thymidine 5'-triphosphate sodium salt 2 dUTP 2'-Deoxyuridine 5'-triphosphate sodium salt 1 CTP Cytidine 5'-triphosphate 2 dGTP 2'-deoxyguanosine...

Xi’an Tianlong Science and Technology Co., Ltd. Biolum ATP, AOAC Research Institute Performance Tested MethodsSM certification number 022401 Table 12. Raw and Calculated Data for the Stainless Steel Matrix Study in Independent Laboratory Study – Doughnut Residue (1) Target Wet Sampling Dry Sampling RLU a b c Sample No. RLU Mean sr RSDr Sample No. RLU Mean

Xi’an Tianlong Science and Technology Co., Ltd. Biolum ATP, AOAC Research Institute Performance Tested MethodsSM certification number 022401 REFERENCES CITED 1. Han, W. and Li, H., Validation Study for the Tianlong Biolum Portable ATP Hygiene Monitoring System for Detection of ATP with ATP Test Swab for Stainless Steel Surface, AOAC Performance TestedSM certification number 022401. Approved February 29, 2024. 2. User Manual – Biolum Portable ATP Hygiene Monitoring System User Manual. Document No. M3001, Version Biolum_Manual_TL_V1.0.002 3. Instruction Manual – Instruction Manual of ATP Test Swab....