YMC-Actus Prep Columns for HPLC and SFC_0621
28Pages

Preparative columns for HPLC and SFC Throughput Yield Long Lasting

Semi-preparative chromatography is the link between analytical HPLC and preparative LC. Even though the chromatographic systems used for (semi-)preparative LC are not as large as preparative LC systems, the objectives remain the same • Purification and isolation of maximum sample quantity • Savings in time and costs. With YMC-Actus, packed with YMC's innovative resins, • Yield • Throughput • Long column lifetime are easily achieved!

YMC-Actus specifications. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 High performance purifications with YMC-Actus (semi-)preparative columns.. . . . . . . . . . . . . . 5 YMC-Triart and Actus technology. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ....

*not all combinations of stationary phase and particle size are available

Actus | High performance purifications with YMC-Actus (semi-)preparative columns High performance purifications with YMC-Actus (semi-)preparative columns How to obtain long lasting columns! YMC-Actus series columns are semi-preparative HPLC columns that have excellent column stability and efficiency as a result of applying axial compression technology. YMC-Actus series columns show high stability under high flow rate or steep gradient conditions which are desirable for milligram scale preparative HPLC of various compounds. Long Lasting ! s Column after 700 runs initial Uniformly high...

Actus | YMC-Triart and Actus technology YMC-Triart and Actus Technology: A perfect match! YMC-Triart is a versatile material with exceptional narrow particle and pore size distributions. YMC-Triart allows challenging pH and high temperature conditions in work day-to-day in laboratories. Most importantly, due to its unique particle composition, a balanced hydrophobicity and silanol activity are achieved which makes YMC-Triart a ”First Choice“ column in method development. Phase overview YMC-Triart C18 versatile applications first choice for method development hydrophobic substances...

YMC Actus I Seamless scalability HPLC/UHPLC to preparative LC ■Hypersil GOLD Gemini NX-C18« XTerra MS C18« ■JgJXSfa- . Triart C8* Triart C18 Symmetry C18« ■SunFireC18 ■Inertsil ODS-3

YMC Actus I Seamless scalability HPLC/UHPLC to preparative LC Evaluation of method transfer performance 0.12 Hypersil GOLD ACQUITY UPLC BEH C18 ^XBridge C18 Eluent: methanol/water (80/20) [Amyl benzene] methanol/water (30/70) [Caffeine, Benzene] With the introduction of UHPLC, sub-2-pm particles became neccessary. Therefore smaller particles have been added to existing column lines. Consequently, sub-2-pm particles may exhibit differences in chromatographic performance. By introducing YMC-Triart, YMC provides matching chromatographic behaviour for all particles sizes! Method transfer HPLC ^...

Actus | Seamless scalability HPLC/UHPLC to preparative LC Secure your method transfer! Differences in selectivity, retention time, and also peak shapes between different particle sizes of commercially available C18 phases in the same brand (or an alternative as recommended by its manufacturer) have been observed. YMC has addressed this issue of method transfer. YMC-Triart columns show identical selectivity and excellent peak shapes for basic compounds for all 3.0 µm to 1.9 µm particle sizes. It allows predictable scale up from UHPLC to conventional HPLC and even to semi-preparative LC, and...

Actus | Effective purification method development ation ific Loading study on an analytical scale Developing a well optimised separation on an analytical scale Scale-up to semi-preparative column dimensions Quality control/proof of concept Purified Product Effective purification method development

Actus | Effective purification method development Development of the analytical method YMC-Triart C18 (5 µm) 150 x 4.6 mm ID 0.01 mg Loading 10 µl injection, 1.0 mg/mL in water AU Impact of loading amount 10.0on peak shape: LOW 15.0 Suitable for purification at high loading Impact of loading amount on peak shape: High min Part No.: TA12S05-1546PTH 0.00 Eluent: A) 20 mM HCOOH for pH5.00 2.7 min 20 mM HCOONH4 for pH 6.7 20 mM HCOONH4-NH3 for pH 9.8 B) acetonitrile 0.5 mg Loading 10 µl injection, 50 mg/mL in water Quality control/proof of concept YMC-Actus Triart C18 (5 µm) 150 x 20 mm ID (x...

Actus | Effective purification method development In order to develop a successful semi-preparative method it is beneficial to carry out the following steps: Developing a well optimised separation on an analytical scale Determine separation conditions by using analytical columns packed with different stationary phases and various conditions. Loading study on an analytical scale Select the particle size of the packing material and the inner diameter of column appropriate for the sample volume. Optimise the separation conditions and perform loadability studies using analytical columns with...

Actus | Separation example Example: Separation of Clindamycin as basic drug Step 1 Development of the analytical method 0.01 mg Loading 10 µl injection, 1.0 mg/mL in water Impact of loading amount on peak shape: High 0.5 mg Loading 10 µl injection, 50 mg/mL in water Impact of loading amount on peak shape: LOW Suitable for purification at high loading 0.40 0.20 Part No.: TA12S05-1546PTH Eluent: A) 20 mM HCOOH for pH 2.7 20 mM HCOONH4 for pH 6.7 20 mM HCOONH4-NH3 for pH 9.8 B) acetonitrile Gradient: 10–75%B (0-15 min) Flow rate: 1.0 mL/min Temperature: 25 °C Detection: UV at 210 nm Pressure:...

YMC Actus I Separation example Loading study on an analytical scale YMC-Triart C18 (5 pm) 150 x 4.6 mm ID 2.5 mg Loading (50 |jL injection, 50 mg/mL in water) Scale-up to semi-preparative scale Flow rate: 1.0 mL/min Scale-up (x100) Flow rate: 18.9 mL/min Temperature: ambient Detection: UV at 210 nm