YMC Capillary Columns for MicroLC and NanoLC
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YMC Capillary Columns for MicroLC and NanoLC - 1

Sensitivity · Selectivity · Robustness YMC Capillary Columns for MicroLC and NanoLC Drug Discovery Life Science Bioanalysis Food Environmental

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YMC Capillary Columns for MicroLC and NanoLC - 2

Capillary Columns Introduction Miniaturisation of liquid chromatography in combination with mass spectrometry has several advantages including improvements in sensitivity, especially at low concentration levels and dramatically reduced solvent consumption, compared to conventional HPLC or UHPLC. With further method optimisation, run time can also be reduced and therefore more solvent or time can be saved. To meet the requirements of MicroLC/NanoLC YMC offers capillary columns especially designed for rapid high-resolution gradient chromatography. Selectivity: • All fully porous YMC-phases •...

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YMC Capillary Columns for MicroLC and NanoLC - 3

YMC Europe GmbH · Schöttmannshof 19 · 46539 Dinslaken · Phone +49 (0)2064 427-0 · Fax +49 (0) 2064 427-222 · Email: info@y

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YMC Capillary Columns for MicroLC and NanoLC - 4

Capillary Columns Columns in micro format YMC MicroLC columns: highest efficiency YMC-Triart C18 MicroLC columns packed with 1.9 μm particles show the highest column efficiency in an efficiency study using van Deemter analysis. Results for YMC MicroLC columns show: • increased resolution • increased separation speed • reduced solvent consumption • reduced cycle times Highest Efficiency: Van Deemter analysis The diagrams show the reduced plate heights (h) of a YMC-Triart C18 MicroLC column together with two other MicroLC columns as a function of the linear velocity at different %B. A low...

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YMC Capillary Columns for MicroLC and NanoLC - 5

15000 - 10000 Theoretical plate number 2.0 1.5 <D o <D -J= I— Temperature: ambient Sample: butyl benzoate Basic compounds Eluent: acetonitrile / water (40/60) The reproducibility of packed columns is shown in terms of theoretical plate number (N) and tailing factor (Tf) for a number of different packed columns. The same is true for YMC capillary columns, which exhibit a very narrow range of variation, also due to the excellent lot-to-lot reproducibly of the packing material. Coordination compounds 1. Hinokitiol (coordination) 2. Methyl benzoate Flow rate: 0.425 mL/min Excellent...

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YMC Capillary Columns for MicroLC and NanoLC - 6

Capillary Columns MicroLC applications Analysis of antineoplastic drugs from wipe samples Standards of 11 antineoplastic drugs time / min Column: YMC-Triart C18 (12 nm, 1.9 μm) 50 x 0.3 mm ID, 1/32" end fittings Part No.: TA12SP9-05H0RU Eluent: A) H2O + 0.1% formic acid B) acetonitrile + 0.1% formic acid Gradient: 10% B (0 min), 50% B (1.6 min), 99% B (2.5 min) Flow rate: 25 μL/min Temperature: 40 °C Detection: Injection: Sample: LC system: SCIEX QTRAP 6500, ESI 4.25 µL (1) gemcitabine, (2) methotrexate, (3) topotecan, (4) irinotecan, (5) ifosfamide, (6) cyclophosphamide, (7) doxorubicin,...

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YMC Capillary Columns for MicroLC and NanoLC - 7

MicroLC applications Wipe samples from different hospital locations Chromatograms for 3 different real wipe samples taken from different locations in a hospital and application areas in hospital wards according to Hetzel et al. The applicability of the developed method according to Hetzel et al. for real samples and the possibility to use this method in the daily life of the pharmaceutical sector is shown [1]. Application data by courtesy of: Thorsten Teutenberg, IUTA - Institut für Energie- und Umwelttechnik e. V., Duisburg, Germany. [1] T. Hetzel, et al., Micro-liquid chromatography mass...

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YMC Capillary Columns for MicroLC and NanoLC - 8

Capillary Columns Column: YMC-Triart Bio C4 (30 nm, 3 pm) 100 x 0.3 mm ID, 1/16" end fittings Eluent: A) H2O + 0.1 % formic acid B) acetonitrile + 0.1% formic acid Gradient: 20 % B (0-2.5 min), 20 %-100 % B (2.5-4 min), 100% B (4-5 min), 20 % B (5-7 min) Temperature: 75 °C, 4 °C Autosampler Detection: Shimadzu LCMS-9030 QTOF Sample: Rituximab dosage form (10 mg/mL, diluted to 0.1 pg/mL) LC system: Shimadzu Nexera Mikros YMC Europe GmbH • Schottmannshof 19 • 46539 Dinslaken • Phone +49 (0)2064 427-0 • Fax +49 (0) 2064 427-222 • Email: info@ymc.de • www.ymc.de

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YMC Capillary Columns for MicroLC and NanoLC - 9

TIC aged solution for patient application Deconvoluted spectrum Column: YMC-Triart Bio C4 (30 nm, 3 pm) 100 x 0.3 mm ID, 1/16" end fittings Eluent: A) H2O + 0.1% formic acid B) acetonitrile + 0.1% formic acid Temperature: 75 °C, 4 °C Autosampler Detection: Shimadzu LCMS-9030 QTOF Sample: Bevacizumab dosage form (10 mg/mL, diluted to 0.1 pg/mL) Bevacizumab solution for patient application (3 mg/mL, diluted to 0.03 pg/mL) LC system: Shimadzu Nexera Mikros Chromatograms of intact monoclonal antibodies, Rituximab and Bevacizumab, both in dosage form. The aged solution (about 6 month old) for...

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YMC Capillary Columns for MicroLC and NanoLC - 10

Capillary Columns f 0 Wheat variety KWS a softwheat # Wheat variety KWS Santiago f 6 Wheat variety kws podium a hard wheat Gluten-Free flour Column: YMC-Triart C18 (12 nm, 3 pm) 100 x 0.5 mm ID, 1/32” end fittings Eluent: A) H2O + 0.1% formic acid B) acetonitrile + 0.1% formic acid Gradient: 5% B (0-1 min), 25% B (6 min), 95% B (8-9 min), 5% B (9.2-12 min) Flow rate: 25 pL/min Temperature: 40 °C Detection: SCIEX 5500 QTRAP, ESI LC system: Eksigent ekspert MicroLC 200 • YMC Europe GmbH • Schottmannshof 19 • 46539 Dinslaken • Phone +49 (0)2064 427-0 • Fax +49 (0) 2064 427-222 • Email:...

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YMC Capillary Columns for MicroLC and NanoLC - 11

MicroLC applications MicroLC-MS/MS analysis of wheat or oat markers for gluten in cookies free Gluten free Gluten & Wheat rs rke o at m a heat Only w s marker It is possible to detect markers in processed food and also distinguish between varieties. In the oat cookies, wheat and oats markers were detected while in the wheat cookies only wheat peptide markers were found. The gluten-free products were actually gluten free, as no markers where detectable. Calibration of gliadin The calibration line obtained from the spiking of gliadin, a specific wheat protein, into gluten-free wheat from the...

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YMC Capillary Columns for MicroLC and NanoLC - 12

Capillary Columns Casein peptide analysis in white wine by HPLC and microLC 5.64 MicroLC YMC-Triart C18 se»sff >2 fold 0.0' YMC-Triart C18 (12 nm, 3 pm) 50 x 0.5 mm ID, 1/32" end fittings (MicroLC) Kinetex XB-C18 (10 nm, 2.6 pm) 50 x 2.1 mm ID (HPLC) TA12S03-05J0RU A) H2O + 0.1% formic acid B) acetonitrile + 0.1% formic acid 2% B (0-2 min), 60% B (8 min), 95% B (8.2-9 min), 2% B (9.1-10 min) 2% B (0-0.3 min), 40% B (4 min), 95% B (4.1-4.3 min), 2% B (4.4-5.5 min) 300 pl/min (HPLC) 25 pl/min (MicroLC) 40 °C SCIEX QTRAP 5500, ESI 10 pl Shimadzu Nexera XR (HPLC) Eksigent ekspert MicroLC 200...

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