VD-200
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ZiVAK FUTURE OF ANALYSIS

x.iVAK TECHNOLOGIES UNIQUE SOLUTION FROM ONE COMPANY ZivakTechnologies is an international company providing ready to use LC-MS/MS and HPLC analysis kits in the clinical diagnostic field. The Research & Development company also manufactures its own fully automated sample preparation and injection systems which enable laboratories around the globe to make efficient use of their chromatography instruments as well as their labor force in a fast, accurate and cost efficient way.

ZiVAiC TECHNOLOGIES Which Diseases Are Associated With Vitamin D Deficiency? • Osteoporosis, osteopenia, osteoarthritis • 17 Varieties of cancer (Including breast, prostate and colon) • Heart disease • High blood pressure • Obesity • Metabolic syndrome and diabetes • Autoimmune diseases • Gout disease • Parkinson’s disease • Depression and seasonal affective disorder • Alzheimer’s disease • Periodontal diseases Which Symptoms Are Associated With Vitamin D Toxicity? Hypervitaminosis D is a state of vitamin D toxicity. Symptoms of vitamin D poisoning include: • Dehydration • Vomiting •...

ZiVAiC TECHNOLOGIES Why Do We Analyze 25-OH Vitamin D2 and D3 Separately? Circulating 25-Hydroxyvitamin D (25-OH-D) is widely recognized as the best indicator of vitamin D status. In blood circulation the two major vitamin d metabolites are 25-Hydroxyvitamin D3 (25-OH-D3) and 25-Hydroxyvitamin D2 (25-OH-D2). 25-OH-D3 is mainly derived from vitamin D3 produced by sunlight in the skin, while 25-OH-D2 is derived from plants in the diet. There are currently two main types of methods used routinely for measuring 25-OH-D: methods based on chromatographic separation followed by non-immunological...

ZiVAX TECHNOLOGIES What Novelty Is VD-200 Bringing to Vitamin D2-D3 Analysis? WALK AWAY SYSTEM HPLC and LC-MS/MS methods are accepted as Gold Standard Methods for separate analysis of 25-OH metabolites of Vitamin D. Chromatographic methods are becoming increasingly widespread in clinical laboratories. The major disadvantage is the time-consuming and laborious extraction procedure that limits the number of samples that can be processed per day. Many of these laboratories cannot use their chromatography systems effectively due to these systems requirement of a qualified staff, complex sample...

ZiVAK TECHNOLOGIES LC-MS/MS Vit D3 C: 5.3 ng/mL Run Time: 1.8 min Calculated LOQ: 3.5 ng/mL What is the Revolution? VD-200 High Sensitivity with Special D-Detector. Zivak Technologies offers higher sensitivity than entry level LC- MS/MS by new design Special D detector based on single wavelength UV. For 25-OH vitamin D3, the limit of detection is 0.74 and the limit of quantitation is 2.22 ng/mL. For 25-OH vitamin D2, the limit of detection is 1.64 and the limit of quantitation is 4.92 ng/mL. The quantiation limit is lower than most entry level LC-MS/MS systems which have quantitation limits...

ZiVAK TECHNOLOGIES Direct Bidirectional LIS Data Transfer From Automated Barcode Reading To Result Zivak VD-200 is especially designed for routine Vitamin D2/D3 analysis in clinical labs. VD-200 enables you to fully automatize your Vitamin D2-D3 analysis and work from primary sample tubes without opening the caps or any human intervention. Zivak VD-200 system automatically generates sample sequence by reading barcodes from primary serum tubes which are barcoded by Hospital LIS system. PLACE YOUR SAMPLES AS YOU GET without aligning the barcodes. Thanks to its rotational barcode reader, the...

ZiVAK TECHNOLOGIES 120 Test Capacity /12 Hours Standart HPLC-UV methods for Vitamin D2-D3 analysis generally take 6-8 minutes. But this 6-8 minutes run time does not include sample transfer from serum tubes, reagent adding, protein precipitation, vortex mixing or centrifugation steps. Because the actual test time exceeds 6-8 minutes, HPLC-UV methods become inconvenient for hospitals and laboratories whose daily samples are more than 50. For this reason, automated and high-throughput chromatography methods are much needed. Zivak VD-200 system gives quantitative, precise and accurate Vitamin...

ZiVAK TECHNOLOGIES calibrator and controls. Because VD-200 system is fully validated with the analysis kit, all validation parameters include variations from automation and analysis. LOD LOQ Accuracy Linearity Intra-Assay Precision (%C V) Inter-Assay Precision (ng/mL) (ng/mL) (ng/mL) (ng/mL) High level Low level Intra-Assay Precision High level Low level High level Low level Inter-Assay Precision High level Low level With an excellent resolution around 150 bar pressure and online SPE module, the VD-200 system can eliminate late peaks In 6 minutes test time. Lower Kit, Maintenance And...

FUTURE OF ANALYSIS KIT Zivak ready to use 25-OH Vitamin D2-D3 UHPLC Analysis kit is used with VD-200 Walk away UHPLC Analyzer. 25-hydroxy metabolites of Vitamin D2 and D3 are extracted from donor horse serum samples using an organic extraction solution after deproteinisation step. Separation was made with an isocratic elution on Zivak 25-OH Vitamin D HPLC column and measured by a special detector which is specially designed for 25-OH Vitamin D2-D3 UHPLC Analysis. Automass Software automatically checks the results with low and high levels of the controls for the intra-day and inter-day...

ZiVAK TECHNOLOGIES TECHNICAL SPECIFICATIONS VD-200 Specifications GENERAL © Power Requirements: - Operating Voltage: 220-230V AC - Operating Frequency: 50 - 60 Hz - Operating Current: 5.7A Max ® DimensionS(W x D x H): 1020mm x 750mm x 840mm ® Working Temperature: 10 - 40 °C ® Storage Temperature: -20 - 70 °C © Humidity: %30 - 80 RH © Sound Level: < 60dB @1 m © Safety: Optional Laser Protection System for user safety. System halts when movement detected inside the instrument. SAMPLING & INJECTION © Sample Capacity: 108 (13 mm Diameter Serum Tube - 5 mL volume) © Loop Vdlume: 20 pi ©...