2019 - 2020 Catalog
129Pages

ZYMO RESEARCH The Beauty of Science is to Make Things Simple

In the past two years, extraordinary new research in the microbiome and metagenome fields have shown a wide range of functions, plasticity, and novel applications. From sequencing DNA on the International Space Station in the Biomolecular Sequencer (BSeq) Mission1, to sequencing thousands of subway stations in the Metagenomics of Subways and Urban Biomes (MetaSUB) project2, to discovering completely novel genera that drive the risks of diabetes in The Environmental Determinants of Diabetes in the Young (TEDDY) project3, many new breakthroughs are constantly being made. These breakthroughs...

Ordering Information Promotional Codes/Discounts Unless specified, promotional codes cannot be combined with any other offers or codes. Sampling Sample kits are available for the evaluation of selected products (see specific product pages on our website: www.zymoresearch.com). Sample kits must be shipped to a valid business or institution address (no P.O. Boxes). Limit one sample kit of each type. Samples take 1-2 weeks for delivery. Bulk Orders/OEM Zymo Research Corporation manufactures most of the products it sells and is pleased to offer discounts on bulk orders including those for OEM...

So, what is epigenetics? The Greek prefix “epi” means “on top of” or “over”, so the term “Epigenetics” literally describes regulation at a level above, or in addition to, those of genetic mechanisms. The field of epigenetics was given its name and a vague definition only 50 years ago, but is now a dynamic and rapidly expanding discipline. Through epigenetics, the classic works of Charles Darwin, Gregor Mendel, Jean-Baptiste Lamarck, and others are now seen in a different light. Today, scientists are using epigenetics to investigate the roles of DNA, RNA, proteins, and environment in...

Quick Product Guide Quick Product Guide Epigenetics Epigenetics Bisulfite Methods Non-Bisulfite Methods Antibody Methods Chromatin Analysis OneStep qMethyl™ Kits High-throughput method for accurate 5-mC detection in genomic DNA. High-throughput method for specific 5-hmC detection in genomic DNA. Protein-DNA interaction analysis. Fast and reliable bisulfite conversion of RNA for methylation analysis. Streamlined procedure for bisulfite conversion of DNA using specialized kits designed for different samples types. Page 12-15 Format: Spin-Column 96-Well Plate Pico Methyl-Seq™ Library Prep Kit...

Epigenetic analyses do not have to be complicated. The scientists at Zymo Research have created this navigation tool to help new and experienced researchers alike tackle epigenetic analysis with ease. Below you will find an overview of some of the most common techniques used for studying DNA methylation with product and service references from Zymo Research to help you along the way. Epigenetics Global Quantification: For understanding complicated changes in the epigenome, the simplest place to start is to determine global changes in DNA methylation. ELISAs are a great way to determine...

Technology Overview: EZ DNA Methylation™ • • • • • Conversion efficiency > 99%. On-column desulphonation and recovery of bisulfite-treated DNA. Conversion workflows in as little as 1 hour. Products available for many sample types, including purified DNA, tissue, cells, FFPE, blood, etc. Recommended as part of Illumina’s workflow. Input Type The gold standard for the analysis of DNA methylation, bisulfite treatment converts unmodified cytosine to uracil while methylated cytosines are protected from this conversion. Sequence analysis post-treatment provides site-specific information on DNA...

DNA Methylation - Bisulfite Treatment of DNA DNA Methylation - Bisulfite Treatment of DNA The complete solution for bisulfite conversion. This all-in-one kit contains: reagents for bisulfite conversion, DNA purification, methylated human DNA with control primers, and a robust hot-start PCR polymerase that is specifically formulated for bisulfite converted DNA. Designed for the first time user requiring a consolidated product to control for bisulfite conversion. Streamlined Process: Ready-to-use conversion reagent is added directly to DNA. Purified bisulfite converted DNA in < 1.5 hours....

DNA Methylation - Bisulfite Treatment of DNA DNA Methylation - Bisulfite Treatment of DNA EZ DNA Methylation-Direct™ Kits EZ DNA Methylation™ Kits • No Purification Necessary: Complete bisulfite conversion of DNA directly from blood, soft tissue, cells, FFPE, and LCM samples. • Low Input: Compatible with small sample inputs, as few as 10 cells or 50 pg DNA. • High Quality DNA: Converted DNA is ready for PCR, Next-Gen Sequencing, and MSP. Epigenetics Description The EZ DNA Methylation-Direct™ Kit is a further refinement of our popular EZ DNA Methylation™ and EZ DNA Methylation-Gold® kits....

RNA Methylation - Bisulfite Treatment of DNA DNA Methylation - Bisulfite Treatment of DNA Visualizing Bisulfite-Treated DNA Bisulfite-treated DNA can be visualized in agarose/EtBr gels following electrophoresis using a standard UVlight source. Now that the bisulfite-converted DNA is single-stranded and has limited base-pairing at room temperature, it is necessary to cool the gel on ice for 5-10 minutes prior to visualization. This will drive some base pairing between the single-stranded molecules and allow recovered material to be visible. Fast and reliable bisulfite conversion of RNA for...

DNA Methylation - Bisulfite Treatment of DNA DNA Methylation - Bisulfite Treatment of DNA Primer Design for Bisulfite and Methylation Specific PCR 1 Frequently Asked Questions Bisulfite-converted DNA can be analyzed by a variety of methods: Bisulfite Sequencing PCR, Methylation Specific PCR, Pyrosequencing, Next-Generation sequencing platforms and many others. The two most common techniques for locus-specific determination of methylation are Bisulfite Sequencing PCR and Methylation Specific PCR. Below is a guide to help you choose the best workflow for your needs: Should the input DNA be...