D5014 / D5014-1 / D5014-2
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Fully Methylated and Unmethylated Control DNAs Human Methylated & Non-Methylated DNA Set • Ideal Positive and Negative Controls: DNA standards, purified from HCT116 DKO cell line, for use as positive and negative controls in methylation-detection applications including bisulfite PCR (BSP) and methylation-specific PCR (MSP) experiments. • Standard Curve Generation: Completely methylated and non-methylated DNA can be mixed together in various proportions to generate a standard curve for suitable quantitation of DNA methylation in experimental samples. • Convenient: Provided with control primers to amplify a fragment of DNA after bisulfite conversion. Meth Meth (+) DNA (-) DNA 100bp Sample 1 Sample 2 Sample 3 Control Control MU MUMUMUMU Example MSP experiment using MSP designed primers for RASSF1. Sample 1 is positive for a Methylated Template. Sample 2 is positive for a Non-Methylated Template and Sample 3 contains Methylated and Non-Methylated Templates. MSP experiment also shows proper controls: Meth (+) DNA Control D5014-2 Human Methylated DNA, Meth (-) DNA Control D5014-1 Human Non-methylated DNA. 2% Agarose Gel, 130V for 35 mins. M = Methylated specific primers, U = Non-Methylated specific primers Human WGA Methylated & Non-methylated DNA Set D5013 1 set Human Methylated & Non-methylated DNA Set D5014 1 set