Rapid Global 5-mC Quantification 5-mC DNA ELISA Kit Accurate Quantification: Sensitive and specific quantification of 5-methylcytosine (5-mC) DNA from a variety of samples. High-Throughput: 96-well format is ideal for processing just a few samples to a large number of samples. Simple: The streamlined workflows can be completed in 4 hours or less. The 5-mC DNA ELISA Kit can quantify 5-mC in numerous DNA samples with close correlation to LC-MS. 100 ng of genomic DNA from mouse brain (MB), mouse kidney (MK), mouse thymus (MT), human brain (HB), human spleen (HS), and E. coli ER2925 were used to coat wells, in triplicate. Percent 5-mC was calculated using the logarithmic equation of the line from the standard curve that was constructed with the Negative Control and the Positive Control. The percent 5-mC calculated in DNA samples using the 5-mC DNA ELISA Kit (ELISA) strongly correlates to mass spectrometry (MS) data of 5-mC found in the respective gDNA sample.