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DNase I Set
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DNase I Set
Product catalog summary
Product Description:
DNase I is an enzyme that cleaves both double-stranded and single-stranded DNA, resulting in 3’-OH oligonucleotides. It is primarily used to degrade DNA in the presence of RNA, making it suitable for applications such as nick translation, random fragment production, genomic DNA cleavage for footprinting, and removal of DNA templates after in vitro transcription. It is also used to remove DNA from RNA samples before downstream applications like NextGen sequencing and RT-PCR. This enzyme is compatible with RNA kits that feature in-column DNase digestion.
DNase I is an enzyme that cleaves both double-stranded and single-stranded DNA, resulting in 3’-OH oligonucleotides. It is primarily used to degrade DNA in the presence of RNA, making it suitable for applications such as nick translation, random fragment production, genomic DNA cleavage for footprinting, and removal of DNA templates after in vitro transcription. It is also used to remove DNA from RNA samples before downstream applications like NextGen sequencing and RT-PCR. This enzyme is compatible with RNA kits that feature in-column DNase digestion.
Specifications:
The product is a recombinant Deoxyribonuclease I (DNase I) set. One unit of DNase I is defined as the amount that increases the absorbance of a high molecular weight DNA solution by 0.001 A260 units per minute per milliliter of reaction mixture at 25°C.
The product is a recombinant Deoxyribonuclease I (DNase I) set. One unit of DNase I is defined as the amount that increases the absorbance of a high molecular weight DNA solution by 0.001 A260 units per minute per milliliter of reaction mixture at 25°C.
Shipping & Storage:
Store the product at room temperature (20-30°C). After reconstitution, keep it on ice until use or store frozen aliquots at -20°C. Avoid repeated freeze/thaw cycles.
Store the product at room temperature (20-30°C). After reconstitution, keep it on ice until use or store frozen aliquots at -20°C. Avoid repeated freeze/thaw cycles.
Instructions for Use:
To reconstitute the lyophilized DNase I at 1 U/µl, add 275 µl of DNase/RNase-Free Water and mix by gentle inversion. For setting up a DNA-free RNA reaction, adjust the sample volume with water or TE buffer to 40 µl, add 5 µl of DNase I, and 5 µl of DNA Digestion Buffer. Mix gently and incubate at room temperature for 15 minutes. Follow with RNA clean-up using products like RNA Clean & Concentrator™-5 (#R1015).
To reconstitute the lyophilized DNase I at 1 U/µl, add 275 µl of DNase/RNase-Free Water and mix by gentle inversion. For setting up a DNA-free RNA reaction, adjust the sample volume with water or TE buffer to 40 µl, add 5 µl of DNase I, and 5 µl of DNA Digestion Buffer. Mix gently and incubate at room temperature for 15 minutes. Follow with RNA clean-up using products like RNA Clean & Concentrator™-5 (#R1015).
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Unit Definition: Shipping & Storage: One unit increases the absorbance of a high molecular weight DNA solution at a rate of 0.001 A260 units/min/ml of reaction mixture at 25°C. Store at room temperature (20-30°C). After reconstitution, place on ice until ready to use or store frozen aliquots (-20°C). Avoid repeated freeze/thawing. Add 275 |jl DNase/RNase-Free Water to reconstitute the lyophilized DNase I at 1 U/pl. Mix by gentle inversion. Reaction setup for DNA-free RNA: Mix by gentle inversion and incubate at room temperature for 15 minutes. Proceed with the RNA clean-up (e.g., RNA Clean &...
Open the catalog to page 2All Zymo Research catalogs and technical brochures
2019 - 2020 Catalog129 Pages
D4303 / D4307 / D43092 Pages
D5325 / D53261 Page
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