#701434 (05-2012) The new ultrafleXtreme
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ultrae treme Beyond Imagination Innovation with Integrity

Explore Your Applications with MALDI-TOF The new ultrafleXtreme™ revolutionizes what you can achieve with a MALDI-TOF and opens new fields for research and industry. Applications such as in-depth characterization of Biopharmaceuticals, high speed tissue imaging based biomarker discovery and glycoproteomics extend beyond classical proteomics utilizing a multitude of technology innovations and unique proprietary software. MALDI Imaging – the complete molecular histology solution from the technology leaders Top-Down Biomarker Discovery New Bottom-Up ImageID™ workflow for protein identification...

More Flexible Than Ever Accurate Intact Protein Molecular Weight Analysis – completing the picture for biopharmaceutical characterization Up to 100x increase of mass accuracy down to the low ppm range for intact protein MW determination due to multiple charged ion signals Bruker DHAP matrix proven for Top-Down analysis producing multiple charged ions Greater than 30,000 resolution for intact proteins due to FlashDetector™ Gold Standard in Top-Down Protein Sequencing Monoisotopic sequence readout of up to 90 residues from N- and C-termini Automated, rapid protein QC reports from BioPharma...

Extreme Performance for Bottom-Up Proteomics The ultrafleXtreme’s next-generation MS technologies enable greater proteome coverage than ever before. The ultrafleXtreme offers unparalleled cutting-edge capabilities for Bottom-Up proteomics at your fingertips. First-class LC-MALDI results Bruker’s 25 years Life Science experience and excellent bioinformatics compliant with HUPO/PSI publication guidelines effortlessly converts unrivalled MALDITOF/TOF data into published results. Identify, quantify and characterize – all your results in the blink of an eye ProteinScape software makes it...

Identification, structural characterization and quantification at a glance File Edit Window Help ka> Quantitation Statist K '% Project Navigator Infoj PrDtei n s & P epti d es |_ Serum albumin precursor - Oryctolagus cuniculus (Rabbit) Myosin-U OS=Oryctolagus cuniculus GN=MYH11 PE=2 SV=2 Sodium channel protein type 9 sub-unit alpha GS=Qryctolagus a „;-■ LC-MS Survey I J_L Specti D Oj-Processing View -trf Sequer ESBAGCEKSL HTLFGDELC?: Vi=L35:T^3~ MA""^"^ 'SJtfiCFLSHX DD5FDLFS1S FEFMri'DS ProteinScape - From data to ■ Data from various workflows (multiple digest enzymes, label or label-free...

Cross the Next Frontier - Glycoproteomics Expertise Delivered The ultrafleXtreme TOF/TOF spectra of N-glycopeptides contain both peptide and glycan information. ProteinScape uses a TOF/TOF specific signature fragmentation pattern generated by intra-ring cleavage of the peptide-binding sugar to assign the aglycone and the glycan molecular weights and triggers their identification in parallel database The specific signature enables the clas- sification and filtering of glycopeptides from non-glycosylated ones in complex cell lysates. ProteinScape integrates the GlycoQuest search engine for...

Ultra rapidcomprehensively ID and 150 kDaglycan structures in MS/MS spectra Easily and product confimation of assign (MOPC21) antibody Representation of an ultrafleXtreme glycoprotein analysis in ProteinScape. All glycopeptides were detected in the LC-MS/MS dataset and the glycan were searched using GlycoQuest. A structure candidate is shown as structure and its fragments annotated to a matching MALDI-TOF/ TOF spectrum permitting manual inspection whenever requested. Ultra rapid product confimation of 150 kDa (MOPC21) antibody Analysis of N-glycosylation pattern present on antibodies...

From Tissue Biomarker Discovery... MALDI Imaging is emerging into an indispensable discovery tool for molecular tissue and histopathological research, reaching out from high resolution imaging tools to statistical cohort analysis and biomarker discovery and identification. Brukers MALDI Molecular Imager solution enables robust and quality-controlled tissue preparations, and links the classical histopathology workflow with the molecular dimension. Unique, patented software and techniques for clinical studies enable powerful biomarker discovery. The ultrafleXtreme is an essential element in...

Ultra rapid product confimation of 150 kDa (MOPC21) antibody A clinical discovery workflow Patient Samples MALDI Images Annotation Inflammation Tumor Connective Tissue Statistical Analysis From beautiful pictures to true biomarker discovery – MALDI Imaging is perfectly suited to analyze biomarkers in tissue samples. Bruker’s unique suite of software tools supports the entire workflow, including statistical analysis. Comprehensive bioinformatic tools for statistical analysis of MALDI Imaging data, such as hierarchical clustering, PCA or pLSA allow researchers to mine data efficiently.

MALDI Imaging covers a multitude of application areas ranging from clinical samples to plant material and animal tissue A: Human bladder cancer specimen; B: Barley seed; C: Rat testis Identification of biomarker candidates are essential for validating results and the translation of MALDI Imaging derived biomarkers to subsequent clinical stages, such as treatment prognosis or survival prediction. Top-Down discovery and identification has been demonstrated successfully*. As well, higher MW protein detection and analysis of formalin-fixed tissues are now enabled by the novel ImageID workflow....

ImageID workflow Ultra rapid product confimation of 150 kDa (MOPC21) antibody Peptide Identification by LC-MALDI Peptide Localization by MALDI-Imaging Protein and peptide list Protein filter list Consecutive sections are processed in parallel to provide both protein distribution and identification simultaneously. A tryptic digest is performed maintaining the resolution of protein distributions. Peptide imaging (right) and LC-MALDIMS/MS (left) yield protein and peptide IDs that is software merged for visualization of protein distribution. 80% of all peptides visible in the images can thus be...