impact II
1 / 12Páginas

impact II Get the full picture the first time Innovation with Integrity

Sensational Capabilities of impact II Optimize your LC-MS methods without compromising performance: impact II delivers the full range of specified performance parameters simultaneously to solve your analytical challenges. The Ultra-High Resolution (UHR) QTOF technology pioneered by Bruker once again defines the standards of what can be achieved using accurate mass LC-MS/MS. New innovations in time-of-flight instrumentation are now available in a robust and market-leading benchtop system. Your Success with impact II Outstanding Hardware Instant Performance Softwa ■ Enhanced dynamic range ■...

Robustness And Simplicity – All Day, Every Day Dynamic range – five orders of magnitude 50 GBit/sec sampling technology enables high Dynamic range acquisition on an LC timescale Greatly increased robustness to sample variation allowing reduced sample pretreatment especially desirable in high throughput quantitative applications. Definitive trace analysis from complex, high-background matrices makes your analytical work more productive. Increased dynamic range, excellent robustness, and full sensitivity gives you the deepest insight into your sample and what might be hiding underneath....

More Dynamic Range, More Accuracy, More Confidence Versatility Sensitive mass transfer from smallest fragment ions to monoclonal antibodies Low mass fragment ion of vanillic acid precursor at 0.1mDa Mass accuracy Sensitivity Patented dual Ion-Funnel: Ruggedness and sensitivity Choice of rugged and sensitive ion sources Mass Spectrum obtained from intact Adalimumab which has a molecule weight of 148k Da “The robustness, sensitivity and spectral accuracy of the impact QTOF-MS has accelerated the process to identify unknown compounds. This is an integral part of long-term goal to ‘sequence’...

TOF with improved Full Sensitivity Resolution (FSR) Spectral accuracy Robustness Market-leading highdynamic range 10 bit detection system New broad mass transfer Quadrupole CID cell with DC gradient Much higher dynamic range in concentration reachable Intens. x107 In-spectrum dynamic range @ real LC speed – don’t miss low abundant peaks.

Productivity and…. Robust proling of large batches of complex biological samples 100 injections of human Urine sample -0.2 ppm mass accuracy True isotopic pattern mSigma mSigma isotopic pattern mSigma mSigma mSigma mSigma mSigma < 15 = unambiguous molecular formula generation even for 45,0 higher m/z values mSigma Value for phenylalanine in 100 urine Injection No. samples measured Peak shapes in urine sample remain constant: Instrument robustness enables comprehensive metabolic profiling studies of large sample sets. Looking at a selected compound: Phenylalanine. SmartFormula provides the...

…Versatility for OMICS research Intens. x105 ∆ ppm/Theory (injection 3) ∆ % abundancy/Theory (injection3) Intact Protein Proling The combination of Isotopic fidelity and mass accuracy that makes the impact II capable of delivering molecular formulae for metabolites is preserved over the mass range. Consequently, even proteins out of a complex mixture (here : overlapping compounds resolved with the Dissect™ algorithm in an E. coli extract) can be extracted and measured with an unrivalled mass accuracy and an exact isotopic pattern. LCMS-89: “High quantification efficiency in plasma targeted...

Qualitative and Quantitative Drug Metabolism Characterization Metabolite Detection Metabolite Detect software compares the data file for the drug (in this case t60) with the corresponding control sample. Auto MS/MS Spectrum A base peak chromatogram of the difference is created allowing metabolites m/z 354, 212 and even 392 to be easily observed. Drug and Metabolite Proles Integration is carried out on the EIC for the measured m/z of each metabolite +/- 0.005 Da. Plotting the ratio of metabolite to internal standard (MIS) vs. time produces the metabolite profiles. Half-life and clearance...

Your Partner in Biopharmaceutical Analysis A powerful benchtop solution with comprehensive capabilities for biological therapeutics screening, characterization and confirmation Intact Mass Measurement of the mAB Adalimumab Rapidly and routinely acquire monoisotopic antibody subunit data, with resolved isotopes and superb mass accuracy. This yields greater confidence in sequence confirmation and easy detection of heterogeneities. Acquire high coverage peptide maps and confidently detect and identify low-level sequence variants with increased MS/MS acquisition speed and wider dynamic range....

One-Shot Full Picture of the Sample ... Full Sensitivity Resolution and high dynamic range are prerequisites to reach extreme low level of detection. High-resolution Extracted ion Chromatogram identifying pesticides with full scan MS and bbCID acquisition in single LC-TOF run. All analytes across the mass range from 70 to 900 m/z are detected. The new HDC collision cell with un-compromised mass-transfer and sensitivity allows for trace level residue screening, confirmation and quantitation. Pesticide screening at 10 ng/L in surface waters Direct injection of 100 μl water: All analytes from...

... for Rapid Profiling and Screening of Unknowns Comprehensive forensic drug screening with maximum confidence in the results with impact II. Accurate mass measurement of ‘diagnostic ions’, including the pseudo-molecular ion, adducts, isotopes as Amphetamine broad band well as CID (bbCID) fragment ions reduces or eliminates false positive findings, even in Amphetamine complex matrices such as (^13C) serum. At trace levels, ‘buried in the grass’ at RT=4.3 minutes, diagnostic fragment Amphetamine Fragm 119 ions m/z 119 and m/z 91 and the 13C isotope for amphetamine are observed....

Dynamic Source Configuration In addition to ESI sources, Bruker life science MS systems support a wide range of source options from Bruker and third-party vendors, all switchable within seconds. CaptiveSpray™ nanoBooster CaptiveSpray nanoBooster is the proteomics ion source that brings your MS to the next performance level – The operation is as easy as electrospray can be. The nanoBooster enables Glycoanalysis, supercharging and pushes up ID rates. Atmospheric Pressure Photo Ionization is used for less polar or non-polar molecules that can not be ionized in either ESI or APCI. APCI...