Excertos do catálogo
Innovating Epigenetics Solutions • DNA and RNA shearing • Tissue and cell disruption • Chromatin shearing • Protein, DNA, RNA extraction • FFPE nucleic acid extraction • Protein aggregation
Abrir o catálogo na página 1Bring epigenetics workflows to new frontiers
Abrir o catálogo na página 2Bioruptor® Diagenode focuses on state-of-the-art preparation of high quality biological and chemical samples by developing the industry’s most advanced water bath sonicators and hydrodynamic devices. Our instruments are ideal for a number of applications in various fields of studies including environmental research, toxicology, genomics and epigenomics, cancer research, stem cells and development, neuroscience, clinical applications, agriculture, and many more. INSTRUMENT for sample preparation REAGENTS AND KITS for optimized shearing FULLY-VALIDATED CONSUMABLES for best performance FULL...
Abrir o catálogo na página 3DIAGENODE BIORUPTOR® / MEGARUPTOR® The Bioruptor is validated by researchers in over 6,000 citations for diverse applications Figure 1. DNA samples of 50 µl were sheared in 0.2 ml microtubes with three different Bioruptor Pico (13 cycles and 30/30 seconds ON/OFF) and analyzed on the Fragment Analyzer (Agilent). The overall average size is 197 bp+/-3.7%. Innovating Epigenetics Solutions Figure 2. DNA samples of 50 µl were sheared in 0.2 ml microtubes with three different Bioruptor Pico (4 cycles and 30/30 seconds ON/OFF) and analyzed on the Fragment Analyzer (Agilent). The overall average...
Abrir o catálogo na página 4They trust us! “My group is mostly focused on epigenetic reprogramming, and I have been using Diagenode products for the last 5 years. My experience with antibodies, ChIP kits and the Bioruptor is nothing but positive. Diagenode products are unique for reproducibility, and this has always been a great plus for the success of my experiments.” Dr. Raffaele Teperino - Environmental Epigenetic Group - Institute of Experimental Genetics, Helmholtz Zentrum Muenchen GmbH, Germany. “High throughput sequencing has dramatically reduced sequencing costs, and the development of duel indexing strategies...
Abrir o catálogo na página 5DIAGENODE BIORUPTOR® / MEGARUPTOR® Bioruptor benefits ROTATION The samples in the tube holder are rotated through the ultrasound field to expose each sample to the same intensity of energy and ensure shearing consistency. The Bioruptor utilizes a sonication bath-based rotor. The walls of the sonication bath reflect the ultrasound waves in a random but reproducible pattern. The Bioruptor sonication bath is equally exposed to ultrasound energy allowing for the dissipation of heat and providing uniform absorption of energy. ADAPTIVE CAVITATION TECHNOLOGY (ACT) Uses a highly-controlled...
Abrir o catálogo na página 6FAST PARALLEL-PROCESSING The design of the tube holders allows you to run up to 16 samples simultaneously in a run of a maximum of 30 minutes. CLOSED ENVIRONMENT The closed tube format prevents the sample from cross-contamination and aerosol formation. ISOTHERMAL TECHNOLOGY A unique cooling system provides isothermal processing to preserve biological samples. FULLY VALIDATED TUBES Diagenode provides specific tubes that ensure maximum energy delivery to samples with minimal attenuation of ultrasound intensity. LOW RUNNING COST < 1€/$/DNA sample
Abrir o catálogo na página 7PAGE 8 DIAGENODE BIORUPTOR® / MEGARUPTOR1 Bioruptor® Pico THROUGHPUT Simultaneous sonication of 6 - 16 samples SAMPLE VOLUME Ultra-low volumes of 20 ^l to larger samples of up to 2 ml TEMPERATURE-CONTROLLED Cooling system with integrated valve* to guarantee the automatic temperature control of the bath during the full shearing process. * Not shown in the picture ♦ DNA CHROMATIN RNA PROTEIN Designed for any researcher Easy and Advanced modes give both beginners and experienced users just the right level of control. Safety is our concern The closed system assures safe use of the Bioruptor....
Abrir o catálogo na página 8Bioruptor® Plus THROUGHPUT Processing of 3 - 12 samples SAMPLE VOLUME Sample size 100 μl – 20 ml TEMPERATURE-CONTROLLED Cooling system with an external valve* to guarantee the automatic temperature control of the bath during the full shearing process. * Not shown in the picture Flexibility in larger sample volumes The Bioruptor Plus is an excellent device for shearing chromatin, cell and tissue disruption and offers a large range of large sample volumes. Use with ease Design simplicity with minimum control of different parameters. Diagenode One THROUGHPUT Sonication of 1 sample per shearing...
Abrir o catálogo na página 9PAGE 10 DIAGENODE BIORUPTOR® / MEGARUPTOR® BIORUPTOR® PLUS The compact bench top device for optimal sample preparation Best suited for cell and tissue sample preparation Chromatin shearing 200 bp - 1 kb DNA shearing 200 bp - 1 kb Chromatin shearing 200 bp - 1 kb DNA/RNA/Protein extraction Mass spectrometry Chemical applications 12 samples (for 0.5 ml tube holder) 6 samples (for 1.5 ml tube holder) 6 samples (for 15 ml tube holder) 3 samples (for 50 ml tube holder) 100 pl (0.5 ml Bioruptor tubes) RECOMMENDED 100 - 300 pl (1.5 ml Bioruptor tubes) VOLUMES 20 and 50 pl 300 pl - 2 ml (15 ml...
Abrir o catálogo na página 10BIORUPTOR® PICO Best suited for ChIP-seq and NGS sample preparation Chromatin shearing 200 bp - 1kb DNA shearing 150 bp - 1 kb RNA shearing 200 bp - 1 kb FFPE nucleic acid extraction Cell lysis and tissue disruption Mass spectrometry Protein aggregation 16 samples (for 0.2 ml tube holder) 12 samples (for 0.65 ml tube holder) 6 samples (for 1.5 ml tube holder) 6 samples (for 15 ml tube holder) 20 - 100 |jl (0.2 ml Bioruptor tubes) 100 jl (0.65 ml Bioruptor tubes) 100 - 300 jl (1.5 ml Bioruptor tubes) 300 jl - 2 ml (15 ml Bioruptor tubes)
Abrir o catálogo na página 11DIAGENODE BIORUPTOR® / MEGARUPTOR® Application versatility FFPE nucleic acid extraction Deparaffinization of FFPE samples is typically performed using a non-polar solvent, such as xylene, or a mineral oil-based method which can be time consuming and messy. Using Diagenode’s Bioruptor is a superior method for removing the paraffin and rehydrating FFPE tissues in just one solvent-free step followed by a mild crosslink reversal to preserve DNA and RNA integrity. Efficient deparaffinization of FFPE sections by sonication with Bioruptor 10 µm sections were sonicated for 3 cycles (30 sec ON/OFF...
Abrir o catálogo na página 12Todos os catálogos e folhetos técnicos Diagenode
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