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Bacteroides Agar

Bacteroides Agar
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Bacteroides Agar(For differentiation and isolation of Bacteroides) Code Directions Suspend 74.0 g of the dehydrated medium in 1,000 mL of distilled water, mix well and heat to dissolve the medium. Sterilize by autoclaving at 121 ° C for 15 minutes, and distribute the medium into Petri dishes. Dry the surface sufficiently before use. It is desirable to use the plate within 3 - 5 hours after preparation. Determinations Differentiation: The medium is able to identify Bacteroides from the organisms that are isolated from clinical specimens and confirmed to be asporogenic gram-negative bacilli of obligate anaerobes. Inoculate the bacilli into GAM Semisolid and incubate for 24 - 48 hours. Take the organisms, streak the surface of the plate, and incubate at 37 °C for 24 - 48 hours under an anaerobic condition. The organisms are identified as Bacteroides, when they grow well on this plate. Selective isolation: When clinical specimens are considered to be lightly contaminated with other organisms, Bacteroides can be isolated selectively by smearing them directly. (Fusobacterium does not grow on this medium, but some aerobic cocci may grow on it). For the quantitative culture, dilute a sample with the following solution (prepared by 1/15 M phosphate-buffered solution, pH 7.2, containing 1 g of polysorbate 80.1 g of L-cysteine hydrochloride and 1 g of agar per liter by autoclaving at 110 °C for 15 minutes). Remarks The medium was devised for the differentiation of Bacteroides. Colistin, neomycin and brilliant green have no influence on the growth of Bacteroides but inhibit Fusobacterium and other bacteria. Asporogenic gram-negative bacilli taken from the clinical specimens are mainly Bacteroides or Fusobacterium. The medium also permits the selective growth of Bacteroides from the clinical specimens. Formula HyServe GmbH & Co.KG, Hechenrainerstr. 24, 82449 Uffing, Germany; www.hyserve.com; info@hvserve.com produced by Nissui Pharmaceuticls co., ltd

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