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ABC of pipette tips

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ABC of Pipette Tip Purity What is contamination and how to avoid it? What are the common contaminants? Deoxyribonuclease that degrades DNA Ribonuclease that degrades RNA What is the source of contamination? What is the effect on samples? Trace metals Also known as pyrogens Lipopoly-saccharides part of cell membranes in Gram-negative bacteria Production Environment Human contact in production Loss of yield in DNA extraction, in PCR and cloning Removal from tips in lab conditions? Sample loss in ctPCR and translational studies Injection molding Causes fever in human and animals Affect growth and well- being of cells in cell-based assays Contaminate all kinds of assays with growth and metabolites False results in PCR / qPCR and cloning False results in trace metal analysis Affect growth and well- being of cells High quality raw material High quality, diamond polished tip molds Clean room manufacturing Automated production without human contact Which product to choose to avoid contamination? High quality standard or filter tips in single tray rack High quality standard or filter tips in single tray rack High quality standard or filter tips in single tray rack High quality sterile standard or filter tips. Standard tips can also be autoclaved for sterility. High quality standard or filter tips in single tray rack High quality tips Questions to ask when choosing pipette tips: What are the certificates of tip purity I need for my workflow? Is the purity of tips tested with low enough detection limits? (DNase, RNase & endotoxin) What is the sterility assurance level of sterile tips? Is the sterility procedure regularly validated? Do the pipettes I use support the purity of my samples? Are the tips & pipettes best fit to prevent leakage & splashing? Are the pipettes easy-to-clean and autoclave? Find out more about contamination free pipetting and other topics in Pipetting Academy webpage! → www.sartorius.com/pipetting-academy

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