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LC Columns and Accessories Catalog 2014-2015

LC Columns and Accessories Catalog 2014-2015
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LC Columns and Accessories Catalog 2014-2015 - 1

Thermo Scientific Chromatography Columns and Consumables 2014-2015

LC Columns and Accessories Catalog 2014-2015 - 2

Thermo Scientific Chromatography Columns and Consumables 2014-2015 As a leader in LC column technology including silica, polymer and porous graphitic carbon manufacturing, bonded phase production and column packing for over 35 years, you can rely on the quality of Thermo Scientific HPLC products. Here we showcase our latest and most comprehensive range of innovative columns, accessories and equipment for fast LC, analytical HPLC and biomolecule separations.

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LC Columns and Accessories Catalog 2014-2015 - 3

Introduction . . . . . . . . . . . . . . . . . . 4-001 Column Selection . . . . . . . . . . . . . . 4-002 Column Protection . . . . . . . . . . . . . 4-016 Columns for Fast and Analytical LC . . 4-022 Columns for Biomolecules . . . . . . . 4-110 LC Accessories . . . . . . . . . . . . . . . . 4-158 LC Equipment . . . . . . . . . . . . . . . . . 4-186 LC Reagents . . . . . . . . . . . . . . . . . . 4-193 LC and LC-MS Instrument Parts . . 4-209 Technical Information. . . . . . . . . . . 4-231 Featured Products Accucore Columns Syncronis Columns Hypersil GOLD Columns Ultimate core performance...

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LC Columns and Accessories Catalog 2014-2015 - 4

LC Column Selection Information in the following section will help you make an informed decision on the appropriate HPLC column for your application, based on stationary phase use, analyte properties, LC-MS requirements or USP specifications. You will also find a useful table of Thermo Scientific phases with specifications, as well as recommended Thermo Scientific alternatives for other popular columns. Refer to the Advanced User Graphic (AUG) on the corresponding product page (illustrated to the right) for more help and information on general purpose column selection. The AUG will show you...

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LC Columns and Accessories Catalog 2014-2015 - 5

Before beginning a new analysis, consider the physical and chemical properties of the analyte(s), the mode of analysis and how the analyte(s) will interact with the surface of the chromatographic phase. To aid column selection, the following guide may be useful. Non-polar Analyte Solubilty Analyte Chemical Properties Recommended Phase Accucore Polar Premium Accucore Phenyl-X Acclaim PolarAdvantage Hypersil GOLD Phenyl Syncronis Phenyl Accucore RP-MS Accucore Phenyl-Hexyl Hypersil GOLD Column Selection Cation exchange / Reversed-phase mixed mode Reversed phase Reversed phase Hypersil GOLD...

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LC Columns and Accessories Catalog 2014-2015 - 6

HPLC Stationary Phase Column Selection continued Polar Analyte Solubilty Analyte Chemical Properties Recommended Phase Thermo Scientific Chromatography Columns and Consumables 2014-2015 Normal phase Hypersil GOLD Silica Syncronis Amino Syncronis Silica Non-polar solvents Hypersil GOLD Amino Syncronis HILIC Accucore Urea-HILIC Cation exchange Syncronis HILIC Polar retention effect on graphite Syncronis Amino Hypersil GOLD HILIC Hypersil GOLD Amino Polar solvents Polar retention effect on graphite Accucore HILIC Syncronis HILIC Anion exchange Accucore Urea-HILIC Polar retention effect on...

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LC Columns and Accessories Catalog 2014-2015 - 7

The Thermo Scientific range offers a broad array of column designs and stationary phases optimized for LC-MS applications. Use the following table to help you choose your column format to best meet your application needs. A variety of HPLC column hardware configurations are available, designed to give superior results for high speed, high sensitivity, high efficiency and convenience. A wide range of particle and monolithic stationary phases allows choices for optimized selectivity. Column Hardware Selection for LC-MS LC-MS Application High throughput analysis Column Hardware Design Javelin...

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LC Columns and Accessories Catalog 2014-2015 - 8

Column Selection for LC-MS continued Packed column selection for LC-MS Monolith columns for LC-MS Analyte Molecular Weight Flow Rate (uL/min Column Hardware PepSwift Monolith ProSwift Monolith ProSwift is also available in larger IDs for high throughput applications.

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LC Columns and Accessories Catalog 2014-2015 - 9

HPLC Column Selection by U.S. Pharmacopeia Specifications* USPCode Description Recommended Phase L1 Octadecyl silane (C18) chemically bonded to porous Acclaim 120 C18 4-061 or ceramic microparticles, 1.5 to 10pm in diameter, Acciaim i300 C18.142 Porous silica particles, 1.5 to 10pm in diameter, Accucore HILIC 4-053 or a monolithic rod Hypersil GOLD Silica 4-036 Octyl silane chemically bonded to totally Acclaim 120 C8 4-061 or superficially porous silica particles, Accucore 4-046' An essentially monomolecular layer of Hypersil GOLD Amino 4-033 aminopropylsilane chemically bonded to totally...

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LC Columns and Accessories Catalog 2014-2015 - 10

HPLC Column Selection by U.S. Pharmacopeia Specifications* continued USPCode Description L17 Strong cation exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the ' hydrogen form, 6 to 12pm in diameter Recommended Phase Page HyperREZ XP Organic Acids 4-156 L19 Strong cation exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the calcium form, about 9pm in diameter HyperREZXP Carbohydrate HyperREZXP Sugar Alcohols 4-156 L20 Dihydroxypropane groups chemically bonded to porous silica or hybrid particles, 1.5 to 10pm...

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LC Columns and Accessories Catalog 2014-2015 - 11

The tables below list Thermo Scientific HPLC sorbents offered for small molecules separations. For Thermo Scientific phases for the separations of biomolecules see page 4-112. Please also refer to the Advanced User Graphic (AUG) for each HPLC phase on the pages indicated. Phase Particle Type Pore Nominal Surface % Endcapping Carbon Size (Å) Area (m2/g) Phase Code Yes Yes Yes Yes Proprietary Yes Yes Yes Proprietary Proprietary Proprietary Proprietary Proprietary Proprietary Yes Yes Yes Yes Yes Yes – Column Selection Acclaim Phases 120 C18 Spherical, fully porous silica 300 C18 Spherical,...

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LC Columns and Accessories Catalog 2014-2015 - 12

LC Columns and Accessories Thermo Scientific Chromatography Columns and Consumables 2014-2015 Thermo Scientific HPLC Phases continued Phase Particle Type Hypercarb Phase Hypercarb Spherical, porous graphitic carbon Hypersil Phases ODS (C18) Spherical, fully porous silica ODS-2 (C18) Spherical, fully porous silica MOS (C8) Spherical, fully porous silica MOS-2 (C8) Spherical, fully porous silica SAS (C1) Spherical, fully porous silica Phenyl Spherical, fully porous silica Phenyl-2 Spherical, fully porous silica CPS Spherical, fully porous silica CPS-2 Spherical, fully porous silica APS-2...

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