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Particle Technology Product Catalog and Technical Reference Guide

Particle Technology Product Catalog and Technical Reference Guide
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Particle Technology Product Catalog and Technical Reference Guide - 1

Thermo Scientific Particle Technology Product Catalog and Technical Reference Guide Magnetic Particles '.$:;:•::'{; Size Standards and Count Control Particles Flow Cytometry Particles Dyed and Fluorescent Particles Clinical Diagnostic and Specialty Application Part of Thermo Fisher Scientific

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Particle Technology Product Catalog and Technical Reference Guide - 2

Thermo Scientific Product Categories Thermo Scientific Particle Technology Magnetic Particles • Sera-Mag SpeedBeads • Sera-Mag Magnetic Particles With a depth and breadth of innovative particle technology design and manufacturing experience that spans over 35 years, we are your proven, reliable source for world class particle technology solutions including: Size Standards and Count Control Particles • Duke Standards • Certified Count Particles Flow Cytometry Particles • Cyto-Cal Calibration and Set-up Particles • Cyto-Plex Surface-Modified Particles for Multiplex Assays Dyed and Fluorescent...

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Particle Technology Product Catalog and Technical Reference Guide - 4

Particle Applications Controls Cv,oCal

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Particle Technology Product Catalog and Technical Reference Guide - 5

ColorRich Blue Color Rich Black Chromo Sphere RD,BLK Particle Applications Air sampling smoke detectors Affinity purification Analytical, sedimentation, separation Biotinylated PCR isolation l cDNA libraries Cell isolation and purification Cell labeling Cell sorting Cell surface markers l l Chemiluminescent assays Contamination control/ flow tracing Cycle sequencing cleanup Dispersion studies Filter checking and testing/ challenge Particle Application Chart Abbreviation Key SA: Streptavidin coated OL: Oligo(dT) coated Gene expression analysis/genotyping l Flow cell focusing l Fluid...

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Particle Technology Product Catalog and Technical Reference Guide - 6

Thermo Scientific Sera-Mag Magnetic Particles Sera-Mag Magnetic SpeedBeads Particles Sera-Mag Magnetic SpeedBeads Protein A/G Sera-Mag Magnetic SpeedBeads Carboxylate-Modified Sera-Mag Magnetic SpeedBeads NeutrAvidin Sera-Mag Magnetic SpeedBeads Streptavidin Sera-Mag Magnetic SpeedBeads Streptavidin-Blocked Sera-Mag Magnetic SpeedBeads Amine-Blocked Sera-Mag Magnetic Particles Sera-Mag Magnetic Carboxylate-Modified Particles Sera-Mag Magnetic Oligo(dT) Particles Sera-Mag Magnetic Streptavidin Particles

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Particle Technology Product Catalog and Technical Reference Guide - 7

Thermo Scientific Sera-Mag Magnetic SpeedBeads Particles Sera-Mag Magnetic SpeedBeads particles respond twice as fast in a magnetic field as our original Sera-Mag Magnetic Particles, and are available in carboxylate-modified, neutravidin- and streptavidin-coated versions, and also as a protein A/G particle. They are especially useful in clinical immunoassays where speed of magnetic response is important, and for isolation from viscous solutions in molecular biology applications. SpeedBeads CarboxylateModified Additional benefits include: • Ideal for demanding applications that require high...

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Particle Technology Product Catalog and Technical Reference Guide - 8

Thermo Scientific Sera-Mag Magnetic SpeedBeads Particles Sera-Mag SpeedBeads Nominal Diameter Bottle Size Binding Capacity Type/ Parking Area Catalog Number Nominal Diameter Bottle Size Binding Capacity Type/ Parking Area Catalog Number Nominal Diameter Bottle Size Binding Capacity Type/ Parking Area Catalog Number Nominal Diameter Bottle Size Binding Capacity Type/ Parking Area Catalog Number Nominal Diameter Bottle Size Binding Capacity Type/ Parking Area Catalog Number Nominal Diameter Bottle Size Binding Capacity Type/ Parking Area Catalog Number Sera-Mag SpeedBeads Carboxylate-modified...

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Particle Technology Product Catalog and Technical Reference Guide - 9

Thermo Scientific Sera-Mag Magnetic Particles World-Class Technology The Sera-Mag family of super-paramagnetic particles are nominal 1 μm magnetic carboxylate-modified base particles (MG-CM) made by a core-shell process. They combine a fast magnetic response time with a large surface area and fast reaction kinetics. • Stable in pH 1 to 13 • Stable in guanidinium thiocyanate • Stable in DMF and DMSO • Stable in sonication environments • Stable in PCR temperature cycling • 60 month shelf-life stability Typically, these particles are used in various molecular biology, nucleic acid isolation,...

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Particle Technology Product Catalog and Technical Reference Guide - 10

Thermo Scientific Sera-Mag Magnetic Particles Sera-Mag Magnetic Streptavidin Sera-Mag magnetic streptavidin (MG-SA) particles contain covalently bound streptavidin pmol/mg), medium (3500 to 4500 pmol/mg) or high (4500 to 5500 pmol/mg) biotin binding capacities. These are measured in picomoles (pmol) of biotin-fluorescein bound per milligram of particle. The multiple levels let you choose the biotin-binding capacity needed to optimize your application. MG-SA can be used as a universal base particle for coating biotinylated proteins, oligos or other ligands to the particle surface. Shelf life...

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Particle Technology Product Catalog and Technical Reference Guide - 11

Thermo Scientific Sera-Mag Magnetic Particles Sera-Mag Magnetic Catalog Number Type/ Parking Area Sera-Mag Magnetic Binding Capacity Bottle Size Nominal Diameter Nominal Diameter Bottle Size Binding Capacity Type/ Parking Area Catalog Number Oligo surface / 0.05% Azide Oligo surface / 0.05% Azide Oligo surface / 0.05% Azide Hybridization, wash, elution buffers Type/ Parking Area Catalog Number Buffer Kit (mRNA isolation) Sera-Mag Magnetic Streptavidin-coated 1% solids Bottle Size Binding Capacity Streptavidin level 3 / 0.05% Azide 3015-2103-011150 Streptavidin level 3 / 0.05% Azide...

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Particle Technology Product Catalog and Technical Reference Guide - 12

Technical Supplement Particle Reagent Optimization: Recommended Adsorption and Covalent Coupling Procedures The following procedure outlines the suggested materials and process for the coupling of Thermo Scientific polymer particles to proteins. These recommended coupling procedures are designed for: Provided here are the protocols for both adsorption and covalent coupling which have proven useful for many of our customers. • Optimal adsorption of proteins to particles • Optimal covalent coupling of proteins to particles • Choice of two protocols for covalent coupling • Simplicity,...

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Particle Technology Product Catalog and Technical Reference Guide - 13

3. Reaction Buffer: MES Buffer 2-(N-morpholino)ethanesulfonic acid: Prepare 10X stock buffer at 500 mM, pH 6.1. The pH will not change significantly on dilution. Store at 4°C and discard if yellowed or contaminated. 4. EDAC 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride 52 µmol/mL: Just before use, weigh approximately 10 mg of EDAC on an analytical balance. For each 10.0 mg weighed, add 1.0 mL of deionized water. Note: EDAC is very sensitive to moisture and undergoes rapid hydrolysis in aqueous solutions. EDAC should be stored in a desiccator at -5°C and brought to room...

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