UNcle
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ITICHAINED LABS

One-stop stability Cracking stability using a pile of one-trick, protein-hungry tools is a ton of work. UNcle combines 3 different measurement modes - fluorescence, SLS and DLS. So you can crank out all your data in just a few hours, and use way less protein. All the info you'll get makes picking the best formulation or protein construct a piece of cake. • Tm & Tagg • Tm with SYPRO (DSF) • AG • Isothermal stability • Thermal recovery • Sizing & polydispersity • Sizing with thermal ramp • B22 • G22 • kD • Viscosity

Get more data with way less protein. The UNi only needs 9 µL of sample, and you pick how you use it. Run 1 sample in the morning, 48 in the afternoon. Do a DLS read if that’s all you need. Or, check DLS, then start a 3-day experiment to monitor real-time stability. Your samples are sealed airtight, so runs can be short or long — your call. Biologics behave differently. With UNcle, you get the whole fluorescence spectrum, so you don’t need to know ahead of time how your protein behaves. No need to add dyes either. UNcle picks up aggregation on two wavelengths — you’ll see both small and...

Uncover way more in one shot Trying new formulations or constructs? Get answers for up to 48 samples in under 2 hours. Measure Tm and Tagg at the same time and know when unfolding leads to aggregation. Add a DLS read before the temp ramp to know if you’ve got aggregate trouble right out of the gate. Unfolding (Fluorescence)

Isothermal stability Dynamic light scattering UNcle handles DLS or SLS readings for days with no sample evaporation. So set your temp and walk away. Get a headsup on long-term stability. Grab polydispersity, radius, and size distribution with amped sensitivity. Then take the same samples and do a thermal ramp just for grins. Intensity (counts/sec) Time (hours)

Get B22 and kD at the same time in the same UNi. Or switch over to G22 for super high concentrations. Learn on the spot if your protein-formulation combo is good to go or risky for aggregation. Use beads to get a snapshot of viscosity without having to wait until later. Figure out how a formulation or concentration tweak changes things with way less protein. Increasing Viscosity Normalized Intensity Concentration (mg/mL)

mCHAINED LABS Unchained Labs 6870 Koll Center Pkwy Pleasanton, CA 94566 Phone: 1.925.587.9800 £ i oriri oiir ooo/i Labs logo, UNcle and the UNcle logo are trademarks and/ T°l|-free: 1.800.815.6384 or registered trademark of U,-chaired Labs. SYPRO is a Email: info@unchainedlabs.com registered trademark of Molecular Probes, Inc.