DisQuE Dispersive Sample Preparation Brochure
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[ QuEChERS Simplified ] QuEChERS Simplified

T he QuEChERS method (an acronym for Quick, Easy, Cheap, Effective, Rugged, and Safe) followed by dispersive solid-phase extraction (d-SPE), is a simple and straightforward sample preparation technique ideal for multi-residue analysis for pesticides, veterinary drugs, and mycotoxins in a wide variety of food and agricultural products. DisQuE dispersive sample preparation extraction products are conveniently packaged with pre-weighed sorbents and buffers in pouches and tubes as described in regulatory methods and protocols.

Compared to traditional sample preparation techniques, DisQuE solutions offer: ■ Decreased time spent on sample preparation ■ Efficient and cost effective sample preparation ■ Reliable, quality product in a convenient format \/Dispersive Sample Preparation

The Science Behind DisQuE To overcome the deficiencies of traditional methods, an improved extraction procedure was developed (Anastas­ iades et s al.)* to extract pesticides from fruits and vegetables. The procedure is based on an initial single-phase extraction using 10 or 15 g sample with acetonitrile at 1 mL acetonitrile per 1 g of sample. A liquid-liquid partition is created by adding excess salts and buffers to the extract. After centrifugation, the acetonitrile layer, containing the pesticide, is collected. The matrix can be further cleaned and the excess water removed, with a single...

[ QuEChERS Simplified ] DisQuE Workflow Simplified QuEChERS Method Sample Preparation Protocol Homogenize sample Transfer sample to 50mL tube Add acetonitrile and internal standard–shake vigorously for 1 minute Add buffering salts and centrifuge Transfer supernatant to d-SPE cleanup tube GC/MS analysis With Large Volume Injection (LVI), analyze the extract directly No LVI, extract is solvent exchanged to toluene LC/MS/MS analysis Dilute the extract with mobile phase 402 Pesticide Residues at 10 ng/g In One 10 Minute Run UPLC® Conditions LC System: Column: Part Number: Column Temp: Sample...

Selection of d-SPE Product for Sample Cleanup Commodity Type Cleanup Provided General Fruits and Vegetables (Celery, Head Lettuce, Melon) Removes polar organic acids, some sugars and lipids Fruits and Vegetables with Fats and Waxes (Cereals, Nuts, Dairy, Avocado) Removes polar organic acids, some sugars, more lipids and sterols CEN Method 15662 25 mg PSA, 150 mg MgSO 4 Part #186004831 150 mg PSA, 900 mg MgSO 4 Part #186004833 25 mg PSA, 25 mg C18, 150 mg MgSO 4 Part #186004832 150 mg PSA, 150 mg C18, 900mg MgSO 4 Part #186004834 Cleaning Up Pigmented Fruits and Vegetables Some fruits and...

[ QuEChERS Simplified ] Ordering Information Kits Name DisQuE Dispersive SPE Kit– Pouch Format (AOAC Method 2007.01) Pouch: 1.5 g sodium acetate and 6 g MgSO 4 DisQuE Dispersive SPE Kit– Pouch Format (CEN Method 15662) 50 mL Tube: Empty 2 mL Tube: 150 mg MgSO 4 and 50 mg PSA Pouch: 1 g trisodium citrate dihydrate, 0.5 g disodium hydrogencitrate sesquihydrate, 1 NaCl and 4 g MgSO 4 50 mL Tube: Empty 2 mL Tube: 150 mg MgSO 4, 25 mg PSA, and 25 mg C18 DisQuE Dispersive SPE Kit (AOAC Method 2007.01) 50 mL Tube: 1.5 g sodium acetate, 6 g MgSO 4 2 mL Tube: 150 mg MgSO 4, 50 mg PSA DisQuE...

T he quality management system of Waters’ manufacturing facilities in Taunton, Massachusetts and Wexford, Ireland complies with the International Standard ISO 9001 Quality Management and Quality Assurance Standards. Waters’ quality management system is periodically audited by the registering body to ensure compliance. Waters, UPLC, ACQUITY, and ACQUITY UPLC are registered trademarks of Waters Corporation. DisQuE and T he Science of W hat’s Possible are trademarks of Waters Corporation. All other trademarks are the property of their respective owners. ©2012 Waters Corporation. Printed in the...